| With the crowd of menopause is becoming larger, palpitations, anxiety and cardiovascular and cerebrovascular diseases, which menopausal women often have, have attached more and more attentions. Menopause is a result of the body’s aging process, and aging is a natural phenomenon. Its main factors are the body itself and environment factor. Mentioned in " Su wen·Tiannina ", "man’s life, centenarian die", it shows that human can live over one hundred years old when we are in a ideal condition, even more than one hundred years old. so the use of natural drugs to slow down the ageing process of human body, reducing the diseases from organs senescence is a very important research. Compound xiang shen di capsules is a compound preparation,its main components are Rehmannia glutinosa Libosch, Eugenia caryophy llata Thunb, Codonopsis pilosula. this product is mainly used for auxiliary alleviate women menopause syndrome,and anti-aging.In compound xiang shen di capsules, clove is the monarch drug, the content determination of preparations take the Eugenol content as standard.Rehmannia glutinosa Libosch, Codonopsis pilosula are taken from Astrotech cream of Jing-yue Complete Works, Ginseng good tonifying spleen lung qi, to treat consumptive internal injuries first to medicine, cultivated land for kidney to medicine, yi Yin blood of top grade, the match, the two drugs a Yin and Yang, as the two instrument.Prescription of clove the application of supercritical CO2 extraction technology, using L9(34) orthogonal method was carried out on the extraction pressure, extraction temperature and extraction time filtering, get the best extraction conditions for pressure: 20 Mpa, temperature: 40 ℃, time: 2 h. With a β- cyclodextrin inclusion of clove essential oil,By L9(34) orthogonal method for volatile oil and β- cyclodextrin, inclusion temperature and the proportion of the filtered closure time, get the best conditions for oil: β- cyclodextrin for 1:8, temperature of 40 ℃, time of 1 hour. Codonopsis pilosula, Rehmannia glutinosa Libosch and clove liquid after extracting with water extraction method to extract effective components, Also using L9(34) orthogonal method to extraction conditions for screening, the best conditions for the material liquid ratio for 1:12, soak for 1.5 hours, extraction of 2 hours, extraction of 4 times. To extract the effective components for enrichment, drying and crushing, Join clove essential oil inclusion compound and add starch and microcrystalline cellulose and other auxiliary materials, using the wet legal system grain of 60% ethanol solution, under the condition of 45 ℃ dry 30 minutes, after granulation, packing get compound xiang shen di capsules.Formulated compound xiang shen di capsules quality standard, including various medicinal materials thin layer identification, routine inspection, using gas chromatography determination of the content of eugenol in preparation for each capsules containing clove on eugenol(C10H12O2), shall not be less than 1.5 mg, and verified methodology. Results: the solvent, negative control solution of eugenol did not interfere, reference substance, the main peak retention time of the test is consistent, a good specificity. Eugenol reference substance of the linear regression equation for y=0.01233x+0.03443, r = 0.9992, the results showed that eugenol in the range of 20.07 to 160.52 ug/ml insider sex is good. This method is simple, accurate, provides a reliable and stable quality standards for compound xiang shen di capsules.Of examining the stability of the preparation, factors affecting test, accelerated test and long-term test, the results show that the product under the condition of high temperature, illumination and humidity change, so the storage should be paid attention to low temperature, light, tight, Conventional stability condition is good. |
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