The Effect Of Fisetin On The Formation Of NO And The Growth Of Vascular Endothelial Cells

Fisetin is extracted from the plant wax tree in the Anacardiaceae wood yellow needle crystal. Studies have shown that fisetin has a variety of physiological functions, including on fibroblast prostaglandin production inhibition, for lens aldose reductase inhibition, anti acetylcholine neurotransmitter role etc.. Further studies on the effects of anti- inflammatory, antioxidant and other pharmacological properties, especially in the treatment of cancer, have a great significance.Vascular endothelial cells are involved in the blood vessels of the blood vessels, and then help to control blood pressure, especially the blood pressure in capillaries. Vascular endothelial cells surfactant can promote blood coagulation. Apoptosis of vascular endothelial cells in the release of necrosis factor can affect the arterial vascular wall cells, leading to atherosclerosis. Endothelial cells are undifferentiated cells that can be differentiated into other types of cells in the growth process. In addition, the endothelial cells of the capillary and lymphatic capillaries can also cause local inflammation or edema.NO can be synthesized and released in some cells and tissues of the animal, and plays a certain biological activity. The synthesis of NO in vivo was generated by Larginine oxidation. L- arginine requires NAPDH and oxygen as a substrate, which can be generated by the NO synthase in the presence of NO. The release of NO is helpful to the relaxation of vascular smooth muscle, which is the most important function of vascular endothelium releasing NO.In order to verify the effect of the ECV304 on the activity of vascular endothelial cells and the ability of NO, we chose the human umbilical vein endothelial cell line to study in vitro. The effect of genistein on vascular endothelial cells was studied by different concentrations of genistein and the effect of genistein.It was found that genistein can inhibit the biological activity of vascular endothelial cells, and the inhibition effect on the concentration of genistein has a certain dependence, with the increase of concentration and time of action, it can inhibit the activity of vascular endothelial cells. The effect of genistein on the formation of vascular endothelial cells NO was similar, and the inhibition was related to the inhibition of AMPK pathway in vascular endothelial cells, but it was not a AMPK/e NOS pathway to determine the formation of NO.The study revealed the relationship between the activity of the vascular endothelial cells of the enamel. It also provides a theoretical basis for the follow-up study, but also for the application in the field of biology and medicine.