A Small Molecular Compound XJ479 Regulates The Balance Of CD4+T Cell Subtypes Through JAK/STAT And NF-κB Signaling Pathway

CD4+T cells can differentiate into Thl, Th2, Thl7 and Treg cells. Thl cells mainly secret IFNy,which promote inflammation. Th2 cells produce IL4,IL5 and IL13, which normally have anti-inflammatory effect. The newly discovered Th17 cells show strong pathogenicity in autoimmune diseases and chronic inflammation. And the regulatory T cells regulate immune response and suppress inflammation and autoimmune diseases.Here we found a small molecule compound XJ479, which effectively inhibit the on set and development of experimental autoimmune encephalomyelitis (EAE) and collagen-induced arthritis (CIA). We analyzed the effect of XJ479 on various subsets of CD4+T cells. Results showed that XJ479 inhibited the production of IL17A and IL17F, and also had a significant role in regulation of the secrection of IL-4, IL13, IL10,etc. Flow cytometry analysis of mice lymphocytes from spleen and lymph nodes showed that Th17 cells was significantly downregulated, whereas Treg was significant increased after treatment of XJ479. At the molecular level, XJ479 regulated proliferation and differentiation of Th17, Thl and Treg through JAK/STAT pathway and NF-kB signaling pathways.In summary, XJ479 effectively regulates CD4+T cell subsets, through JAK/STAT pathway and NF-kB signaling pathway.