Effects Of Mesoporous Bioactive Glass With A Hierarchical Pore Structure On Proliferation And Differentiation On Osteoblasts In Vitro

Mesoporous bioactive glass with a hierarchical pore structure(MBG), like bioactive glasses,had been proved that it can make great effect on osteoblast on proliferation and differential.Meanwhile,it has both macroporous and mesoporous.Mesoporous can be loaded with a variety of small molecule drugs and control drug release effect,therefore it can be used for drug delivery to target cells;macroporous can connect into a network, that allows cells to grow into the channel and space, to guide the bone cells grow in the wall of the hole, and to transport nutrients to grow new bone tissue.In recent years,it has attracted increasing interest due to its extraordinary functionalities.Compared with the artificial templates, the biological templates, with low-cost, abundant, renewable and environmental friendly advantages, own sophisticated structures and morphologies.Mesoporous bioactive glass scaffolds with a hierarchical pore structure using natural sponge as biotemplate was designed and prepared by the College of Food Science and Engineering,Ji Lin University. Objective:The aim of our study was to investigate the effects of mesoporous bioactive glass with a hierarchical pore structure on proliferation and differentiation on osteoblasts in vitro through MTT method,PNPP method,Real time PCR method. Methods:1.MG63 osteoblast-like cells were cultured with leaching liquor of hierarchical porous bioactive glasses for 1,3,5d to analyze proliferation on MTT methods.2.PNPP method was used to assay ALP activity of osteoblasts.3.Real-time PCR was used to detect the m RNA expression of Runx2,Sp7 for Results:On the 1st,3rd,5th day of cell cultivation,the proliferation of MG63 in MBG group was all significantly increased(P < 0.01) and the activity of ALP was significantly increased too(P<0.01).Real-time PCR showed that the expression of Sp7 m RNA in MBG group was significantly up-regulated(P < 0.01),while the expression of Runx2 was no significant difference. Conclusions:MBG presented positive effects on proliferation and differentiation on osteoblasts in vitro,compared to normal bioactive glasses.