Effects Of26RFa On Human Mesenchymal Stem Cells Differentiating Into Osteoblasts

Objective:To observe the effects of26RFa on the proliferation and differentiation of human bone marrow mesenchymal stem cells (Human Mesenchymal Stem Cells-bone marrow, HMSC-bm).Methods:In order to obtain the most efficient concentration of26RFa, Cells were detected by MTT analysis. Cells were planted in6cell plate and which were inoculated with six plates (two plates with coverslip), each plate cells were divided into experimental and control groups. Experimental group contained26RFa(10-11mol/L), the control group without26RFa. After had been treating8days、12days、16days, each plate cells of osteoblasts-induced group were identified by ALP activity analyses. Then calcium deposition, after had been treating21days^28days, were detected by Alizarin red staining and Von Kossa staining in the cell of osteoblasts-induced group. The expression of cbfal protein was detected by Western blot.Results:When the cells had been induced by osteogenic inducer during8days,12days and16days respectively, alkaline phosphatase activities in experimental group raised greatly compared with control group (P<0.05, P<0.01, P<0.05). When the cells had been induced by osteogenic inducer during21days、28days respectively, the calcified nodules in experimental group increased in contrast with that in control group. The expression of cbfal protein in experimental group significantly increased compared with control group (p<0.05).Conclusions:26RFa can promote the differentiation of Human Mesenchymal Stem Cells into osteoblasts under appropriate culture condition.