Research Community Structure Of Intestinal Flora In Patients With Type â…¡ Diabetes

With imbalance of modern people’s dietary structure, human health is threatened seriously, especially the incidence rate of metabolic diseases such as type 2 diabetes increases rapidly. Medical research showed the gut microbiota is closely related to the occurrence of type 2 diabetes, so the specific relationship between the two has become a hot topic of research in the field of science.This study compares the differences of diversity and community structure on all bacteria, Clostridium leptum and Bacteroides of human intestine in type 2 diabetes with normal people, and whether with the drug treatment, whether with family inheritance and whether with concomitant obesity cases in type 2 diabetic patients by using the method PCR-DGGE. To further explore the effects of the above factors on the changes of human intestine microflora. Besides, we also studied the how much content and variation situation of Bacteroides、Bifidobacterium、Clostridium leptum and Lactobacillus in the intestinal tract of the above group of populations by using the method real-time fluorescent quantitative PCR, which opened up a new world for defensing and healing the type 2 diabetes in the future.We analyzed the differences of microbial community structure between the above each group of populations by PCR-DGGE、principal component analysis and diversity statistics method. The results showed that the drug treatment, genetic history and obesity had an important influence in bacterial community structure, and the bacterial community structure in the group of drug treatment was more close to the normal population than in other groups of patients with diabetes.Specific fragments of four kinds of important flora were amplified with the fluorescence quantitative PCR. Differences and changes of the above four kinds of flora were studied. The relative contents of Bacteroides and Bifidobacterium in healthy group were much lower than type 2 diabetes groups, but the relative contents of Clostridium leptum and Lactobacillus in healthy group were higher than type 2 diabetes groups. Among the type 2 diabetes groups, the contents of Bacteroides and Bifidobacterium after drug treatment were lower than non-drug treatment, while the relative contents of Clostridium leptum and Lactobacillus are higher. Contents of Bacteroides and Bifidobacterium in obese patients accompany with high blood glucose were lower than alone high blood glucose patients, while the relative contents of Clostridium leptum and Lactobacillus of the obesity are higher. Genetic inheritance has no statistical significance. These four gut flora are closely related to the origination and development of diabetes. The contents of four kinds of important intestinal flora in drug treated patients are closer to healthy group. Obesity aggravates diabetes.In conclusion, the community structure of gut flora in type 2 diabetes was very different with normal people. The obesity with the type 2 diabetes would aggravate the imbalance of gut flora. The drug treatment would recover the imbalance partly. The results of this topic research suggest: diabetes and intestinal flora structure of interdependence and mutual influence. Thus, to prevent and treat of metabolic diseases such as type 2 diabetes by adjusting and improving the community structure of intestinal.