Dynamics Of Bifidobacterium And Lactobacillus In The Intestinal Of Obese Rats

AIMTo investigate the intestinal dynamics of bacteria strains Bifidobacterium and Lactobacillus in obesity rats.METHODSBoth Bifidobacterium and Lactobacillus were labeled by five-(and six-)carboxyl fluorescein diacetate succinimidyl ester (CFDA-SE) molecular probes and fed in amout of 4 mL×109 CFU/mL for all groups. Faeces were took at 0,2,4,8,12,24,48,72,96 h respectively after orogastric administration, rats were sacrificed at 2,4,8,12,24 h respectively from duodenum, jejunum, ileum, and colon. The number of fluorescence positive bacteria were observed under the fluorescence microscopic.RESULTS1.There was less Bifidobacteria in faeces of obese group at 2,4,24 h than normal food group, also the number of Lactobacillus at 2,12,24 h was less than normal food group(P<0.05).2. Bifidobacteria in obese group at 4h, normal group at 2,4,8,24 h showed significant difference in fluorescence examination from Lactobacillus group (P<0.05).3.The distribution of Bifidobactenum in Obese group was different from normal food groups at duodenum on 2,12 h, jejunum on 2,8 h,Ileum on 2,4,8,12 h, and Colon on 2,4,12,24 h (P<0.05); Also Lactobacillus were different from normal food groups at jejunum on 2 h, Ileum on 8,12 24 h, and Colon in 2,12,24 h (P<0.05).CONCLUSION1.The Intestinal motility is different with the fed of Bifidobacterium and Lactobacillus.2.Obesity could influence the intestinal motility in fed of Bifidobacterium and Lactobacillus.