Correlations Between Butyrylcholinesterase And Blood Lipids In Hyperlipidemia Rabbits

Butyrylcholinesterase(Bu Ch E) is a serine hydrolase, which is abundant in human serum. None of certain conclusion could be obtained to confirm the relationship between Bu Ch E and blood lipid metabolism. We firstly use Hyperlipidemia Rabbits, specific Bu Ch E inhibitor R-bambuterol hydrochloride(R-BMB) and a good lipid-lowering drug atorvastatin calcium(ALF) to study the correlations between Bu Ch E and blood lipids. The concrete research content is as follows:1. Correlations between Bu Ch E and blood lipids in hyperlipidemic rabbits(1) Animal grouping, sampling and determining: normal group(n=6) and high lipid group(n=24). High lipid group was randomly divided into control group, R-BMB 2mg group, R-BMB 10 mg group and ALF 2 mg group, after inducing the rabbits forming hyperlipidemia(Peripheral blood was colleted during this process). The four groups were treated with normal saline, R-bambuterol 2mg/kg, R-bambuterol 10mg/kg and atorvastatin calcium 2mg/kg by intragastric administration respectively every day. The peripheral blood was collected at 4th week after administration. Then all rabbits were sacrificed by anaesthetization, and tissue samples were collected. Serum Bu Ch E activity was determined using the Ellman’s method. TC, TG, LDL-C and HDL-C were determined.(2) Changing of blood lipid and Bu Ch E activity: Comparing with normal group, TC, LDL-C and HDL-C of model group were significantly increasing with time. Accordingly, at 8th week, they were 22.09, 33.03 and 8.88 times higher than that of 0th week. From 0 to 10 week, there is not significant change in Bu Ch E activity. While, at 14 th week, Bu Ch E activity of model group was 1.22 times(P <0.05) that of its at 0th week. At 4th week of administration, comparing to model group: in R-BMB 2mg group, TC was 68 % of pre-administration(P <0.01), Bu Ch E activity was 45 % of pre-administration(P <0.01); in R-BMB 10 mg group, TC was 77 % of pre-administration(P <0.01), TG 55 % of pre-administration(P <0.01), LDL-C was 63 % of pre-administration(P <0.01), Bu Ch E activity was 24 % of pre-administration(P <0.01);in ALF 2mg group, TC was 84 % of pre-administration(P <0.01), TG was 65 % of pre-administration(P <0.05), Bu Ch E activity was 71 % of pre-adminstration(P <0.01).(3) Correlations: Results showed that, high-cholesterol diet can significantly increase TC, LDL-C, HDL-C level(P <0.01), Bu Ch E activity didn’t change significantly(P >0.05); R-BMB can inhibit Bu Ch E activity, blood lipid changed significantly. Specially, high dose of R-BMB(10 mg/kg) decreased TC, TG, LDL-C significantly(P <0.01); ALF decreased TC(P <0.01) and TG(P <0.05) significantly, as well as Bu Ch E activity(P <0.01). These results showed that, increasing blood lipid is not the occasion to changing Bu Ch E activity, while inhibit Bu Ch E might lead to lower blood lipid, and the underlying mechanism needs to be further investigated.2. Bu Ch E, including the bounded and unbounded on the membrane, was extracted via homogenized from liver, brain and heart and its activity was determined. The results showed that the Bu Ch E activity of control group is 1.41 times higher than that of normal group(P <0.05)3. In this paper, CHO cell and Pichia pastoris were used to express Bu Ch E. Bu Ch E was not detected in the Pichia pastoris culture broth. The yield of Bu Ch E expressed in CHO culture was about 30 μg/L.