The Role And Mechanism Of Ginkgo Biloba Extract On Intervention In Human Embryonic Lung Fibroblast

Objective: The interstitial pulmonary fibrosis is a refractory disease of respiratory system, and there is no ideal therapeutics at present, Clinical observation on the effects of Ginkgo biloba extract in the treatment of idiopathic pulmonary interstitial fibrosis shows that has certain curative effect.Through the observation on the effect of Ginkgo biloba extract on extracellular matrix,inflammatory cytokines,the cell proliferation and apoptosis of human embryo lung fibroblasts to explore its mechanisms.Methods: Order human fetal lung fibroblasts as the research object were randomly divided into five groups:normal control group,model group, Ginkgo biloba extract low-dose group, Ginkgo biloba extract medium-dose group and Ginkgo biloba extract high-dose group,human fetal lung fibroblasts were added to the normal control group, TGF- β1 was added to the model group when cultured human embryo lung fibroblasts.On the basis of the model group,different concentrations of Ginkgo biloba extract were added to the experimental group.The drug concentrations were 5ul/m L,10ul/m L, 20ul/m L.The cells were cultured in constant temperature incubator.1.The effect of Ginkgo biloba extract on the proliferation and apoptosis of human embryonic lung fibroblasts,the lung fibroblast cells were treated by the MTT test. Absorbance(OD) was measured at wavelength 490 nm.The apoptosis of cells were tested by the flow cytometer detection.After drug effected 24 h,The apoptosis rate of human fetal lung fibroblasts was measured.The above data were analyzed,P < 0.05 as there was a significant difference.2. The effect of Ginkgo biloba extract on the extracellular matrix of human embryo lung fibroblasts,the contents of Col- I, Col- III and α-SMA protein in human embryonic lung fibroblasts were detected by ELISA test.The contents of m RNA of the Col- I, Col- III and α-SMA in human embryonic lung fibroblasts were detected by RT-PCR. The above data were analyzed,P < 0.05 as there was a significant difference.3. The effect of Ginkgo biloba extract on antioxidant activity of human embryo lung fibroblasts,the contents of ECSOD protein and m RNA in human embryonic lung fibroblasts were detected by ELISA and RT-PCR.4. The effect of Ginkgo biloba extract on the inflammatory cytokines of human embryo lung fibroblasts,the contents of PDGF protein and m RNA were measured.The above data were analyzed,P < 0.05 as there was a significant difference.Results: 1.The extract of Ginkgo biloba significantly inhibited the proliferation of human embryonic lung fibroblasts and promoted the apoptosis of human embryo lung fibroblasts.The experimental results of MTT showed that cell proliferation OD value decreased significantly(P<0.05)between the experimental group and the model group.With the increase of Ginkgo biloba extract concentration, cell proliferation was significantly decreased.Flow detection results showed that the extract of Ginkgo biloba promoted the apoptosis of human embryonic lung fibroblasts.2.Ginkgo biloba extract inhibits the synthesis of human embryonic lung fibroblast matrix,compared with the model group,the contents of substance such as Col- I, Col- III,α-SMA and m RNA were decreased obviously(P<0.05).3.Ginkgo biloba extract can enhance the antioxidant capacity of cells.ELISA test and RT-PCR detection results show that the contents of ECSOD protein and m RNA in human fetal lung fibroblasts increased significantly in the experimental group compared with the model group(P<0.05).4.Ginkgo biloba extract reduces the secretion of inflammatory cytokines in human embryo lung fibroblasts,ELISA test and RT-PCR detection results showed that the contents of PDGF and m RNA in the experimental group were significantly decreased compared with the model group(P<0.05).Conclusion: Ginkgo biloba extract can effectively inhibit the proliferation of human fetal lung fibroblasts caused by TGF-β1,and promote the apoptosis of human embryonic lung fibroblasts.Ginkgo biloba extract can improve the human embryonic lung fibroblast cells antioxidant capacity,and to reduce the amount of inflammatory cytokines.