The Protective Effect Of Ginkgo Biloba Extract On Donor Lung In Lung Transplantation In SD Rats

Objective: Ginkgo biloba extract contained several effective components, of which the flavonoid glycosides and terpene lactones act the main pharmacological effect of this drug. Many researches have confirmed that it act as a scavenger of free radical and antagonist of platelet activating factor(PAF), which plays a very important role in the mechanism of ischemic reperfusion injury in lung transplantation. Our study is aim at examining the protective effect of standardized extract of Ginkgo biloba (Egb761 Dr. Willmar Schwabe GMBH &Co. flavonoidglycosides :3.5mg/ml ; terpene: 0.21mg/ml: Ginkgolic acid<5ppm) on donor lung in lung transplantation in SD rats.Methods: SD rats orthotopic left lung transplantation model were employed. Group I (n=5): donors received no drug pretreatment and perfused with cold normal saline(4 *C) , after 2 hours of cold ischemia, donor lung wastransplanted to the recipient. Group n (n=5): Donors pretreated with Egb761(0.15ml/100g body weight) 30 minutes before harvest and another dosage of Egb761 (Iml/lOOg body weight) were added to the perfusate, and the recipients also pretreated with Egb761(0.15ml/100g body weight) 30 minutes before operation. Malondialdehyde (MDA) and Glutathione (GSH) were determined in blood serum 0.5 and 1 hour after reperfusion and in lung tissue after 1 hour of reperfusion, lung wet/dry ratio was measure after 1 hour of reperfusion. We take another 5 rats , MDA and GSH in blood serum ^ lung tissue and lung wet/dry ratio were determined after anesthesia as performed in Group I and II. This data served a base line of rats before ischemia.Results: 30 minutes after reperfusion the blood serumMDA concentration in Group I is higher than in pro-ischemia(PO.001) and in Group II (PO.001); GSH in Group I is lower than in pro-ischemia (PO.001) and in Group II (PO.001). This change became much worse after 1 hour of reperfusion. In lung tissue, MDA and GSH concentration are significant difference in Group I (MDA: PO.001, GSH: PO.001) and in Group H (MDA: PO.001, GSH: P=0.003) after 1 hour of reperfusion. We also observed a significant difference between two Groups(MDA: PO.001; GSH: P=0.01). Lung water gain(lung wet/dry ratio) is higher in Group I (P=0.001) and in Group II (PO.001) as compared with in pro-ischemia, and between the two Groups, Group II is higher than in Group I (P=0.001).Conclusions:1): Lipid peroxidation is one of the mechanism in lung ischemic reperfusion injury in lung transplantation.2): The protective effect of Egb761 is by inhibiting on lipid peroxidation.3): Egb761 partially reduced donor lung ischemic reperfusion injury in our study.4): The mechanism of Egb761 on lung edema is unknown and need to be investigated.