| Purpose: Based on the study of senile dementia " Xuan Fu " theory of TCM, by choosing associated with xuan fu function nature of MDA, SOD and other active enzyme and β-APP m RNA conducts research objective.Through Yizhi Tongxuan Decoction intervention on hippocampal injection of β-amyloid 25-35 dementia model is set up, Using modern molecular biology detection method objective proof " xuan fu " and the correlation between alzheimer diseas0.e(AD), to explore open after " xuan fu " for A model rats had the beta and MDA, SOD, β-APP m RNA transcription, And conducting clinical research For dementia diagnosis and treatment from the viewpoint of "fu xuan" provide new method and train of thought. Method: Select > = 10 months 90 type male Wistar rats, only through the injection of hippocampus A beta 25-35 preparation dementia model, and the rats randomly divided into educational tong xuan tang high, medium and low dose group, the more nai pp group, control group and model group. Lavage after 6 weeks, anesthesia executed in rats, to detect the hippocampus, using immunohistochemical techniques to detect model mouse tissues expressed A beta, MDA, SOD content in hippocampus is measured by ELISA method, real-time fluorescent quantitative PCR technique to detect beta APP expression m RNA transcription.The clinical curative effect on senile dementia compared by setting the control group, Tong Xuan Yizhi Decoction has a better curative effect. Results: Yizhi Tongxuan Decoction can reduce dementia model mouse tissues A beta expression and MDA content, increase SOD content, and can restrain the beta APP expression m RNA transcription.Have certain curative effect on patients with Alzheimer’s disease in clinic. Conclusion: Yizhi Tongxuan Decoction can reduce dementia model mouse tissues A beta expression and MDA content, enhance SOD content, inhibit the beta APP expression of m RNA transcription, dialectical treatment of AD by xuan fu has the feasibility. |
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