Study On Metabolomics Of Thermal Injury Kidney Collaterals Syndrome Of Henoch-schonlein Purpura Nephritis In Children

Objiective:This research attempts to verify prophase research results that TCM syn drome typing of Henoch-Schonlein purpura nephritis(HSPN) in children is reasonable b ased on metabonomic research mentality and technologies; definite the changes in uri ne metabolic fingerprinting of HSPN compared with health people,and to explore bio markers(group) of HSPN;definite the changes in urine metabolic fingerprinting of therm al injury kidney collaterals syndrome of HSPN compared with health people,and to ex plore biomarkers(group) of thermal injury kidney collaterals syndrome of HSPN.To exp lore material basis and diagnostic of thermal injury kidney collaterals syndrome of HS PN based on metabbolomics levels.Method:Collecting and storing fasting morning urine samples of 9 chidren sufferin g from thermal injury kidney collaterals syndrome of HSPN and 9 chidren suffering fr om HSPN but not thermal injury kidney collaterals syndromed and 22 health chidren.T hen scanning spectogram of these samples by UPLC-LTQ-Orbitrap to obtain metabolic fingerprinting.After pretreat spectra,obtaining the three-dimensional datas with retention t ime,mass to charge ratio and the peak area of metabolites.studying overall of these sa mples distribution by principal component analysis(PCA),further then comparing these d ifferent groups by orthogonal partial least squares discriminant analysis(OPLS-DA).Final ly getting otherness components from loading scatter plot and variable important in pro jection(VIP)of OPLS-DA,and screening these datas with T-test.these components maybe the"biomarkers(group)".Result:â‘ There are obvious distinction between HSPN and control group on scores scatter plot of OPLS-DA.â‘¡There are obvious distinction between thermal injury kidne y collaterals syndrome of HSPN and control group on scores scatter plot of OPLS-DA. â‘¢Analysis on Human Metabolome Database(HMDB) and Kyoto Encyclopedia of Gene s and Genomes(KEGG),the"biomarkers(group)"of HSPN may be Tyrosol 4-sulfate,Argin yl-Serine,Estrone,Tryptophyl-Methionine,Vitamin K2,Glucoobtusifolin,S-aminomethyldihydr olipoamide,Phenylalanyl-Arginine,Histidinyl-Valine,L-Methionine,Homocysteine thiolactone, Phosphatidylcholine,Cholestane-3,7,12,25-tetrol-3-glucuronide,Acetoacetic acid,N2,N2-Dime thylguanosine;the"biomarkers(group)"of thermal injury kidney collaterals syndrome of H SPN may be Propionic acid,Aldosterone,Homo-L-arginine,itaminK2,Glucoobtusifolin,S-am inomethyldihydrolipoamide,Phenylalanyl-Arginine,L-Methionine,Homoc-ysteine thiolactone.Conclusions:â‘ Mrtabolomics research method can successfully distinguish between HSPN group and normal control group,the"biomarkers(group)"may be Tyrosol 4-sulfate, Arginyl-Serine,Estrone,Tryptophyl-Methionine, Vitamin K2, Glucoobtusifolin, Saminomet hyldihydrolipoamide, Phenylalanyl-Arginine, Histidinyl-Valine, L-Methionine, Homocyst eine thiolactone, Phosphatidylcholine, Cholestane-3,7,12,25-tetrol-3-glucuronide, Acetoac etic acid, N2,N2-Dimethylguanosine.â‘¡Mrtabolomics research method can successfully di stinguish between thermal injury kidney collaterals syndrome of HSPN group and norm al control group,,the"biomarkers(group)"may be Propionic acid,Aldosterone,Homo-L-argin ine,itaminK2,Glucoobtusifolin,S-aminomethyldihydrolipoamide,Phenylalanyl-Arginine,L-Methionine,Hom ocysteine thiolactone.â‘¢The prophase research results that TCM syndrome typing of Heno ch-Schonlein purpura nephritis(HSPN) in children is reasonable.