Research Of PLLA/Gelatin Nanofibre Scaffold’s Cytocompatibility

Objective: Prepared by Electrospinning PLLA/gelatin (PLLA/Gelatin) nanofiber scaffold and chondrocytes,and evaluate itsbiocompatibility and explore its feasibility as a cartilage tissueengineering scaffolds for tissue engineering technique,in order toprovided repairing cartilage lesions experimental basis.Methods: First, in accordance with the PLLA and Gelatin massfraction ratio of3:7ratio join configured HFIP/DMF (98:2) solution,and the configured final concentration of10%of the spinning solution. Ina particular operating parameters (operating voltage15kV, accept distance18cm, flow rate syringe pump pushing the liquid in the syringe is30μL/h, air humidity around60-75%, room temperature about25℃), the useof electrostatic spinning preparation device PLLA/Gelatin nanofiberscaffold. Stent surface morphology by scanning electron microscopy(SEM) analysis of the prepared, the diameter distribution. Extraction ofrabbit articular cartilage cells and passaged to the second generation ofthe chondrocytes seeded sterilized stent, the stent was observed in thedistribution of chondrocytes adhering by DAPI fluorescent staining, withdiphenyl tetrazolium bromo salt (MTT) on the bracket cell proliferationassay, and by HE staining and Alcian blue-nuclear fast red staining andthe ability to observe the growth of cartilage cells in the scaffold matrixsecretion of cartilage-specific cells.Results: PLLA was prepared by electrospinning of/Gelatin stand for three-dimensional porous structure of interconnected pores rate, the gapbetween the good, smooth fiber, fewer adhesions occur, distributed indiameter between0.7μ m-1.0μ m The average diameter of0.833um. DAPI staining was observed second chondrocytes can be seenon the carriage stable adhesion growth. MTT assay showed that thesecond generation of chondrocytes capable of PLLA/Gelatin stent stableproliferation, which gradually approaches the growth plate as a positivecontrol group of cells, and the cells better than pure PLLA bracket. HEstaining and Alcian blue-nuclear fast red staining of cartilage cells cansecrete cartilage-specific extracellular matrix in the PLLA/Gelatinbracket, and cartilage and cartilage lacuna sac-like structure.Conclusion: Chondrocytes can lay on electrospinning PLLA/Gelatin three-dimensional porous nanofiber scaffold adhesion,proliferation and secretion of cartilage-specific extracellular matrix,scaffold has good biocompatibility, tissue engineering scaffolds forcartilage repair cartilage lesions provides experimental in accordancewith.