The Research Of The Biocompatibility Of Gelatin/poly ε-caprolactone Composite Nanofibrous Scaffold

Objective 1. To study the effects of the Gelatin/polyε-caprolactone(PCL)composite nanofibrous scaffold on wound healing of full-skindefect on rabbits; 2. To study the biocompatibility of Gelatin/PCLcomposite nanofibrous scaffold cocultured with BMSCs in vitro,andprovide certain experimental basis for further reseach in skin tissueengineering.Methods 1. full-skin defects(Φ=3.0cm) were covered with Gelatin/PCL composite nanofibrous scaffold,PCL nanofibrous scaffold asexperimental groupl,group2 and gauze as control group.Wound healingrate was separately calculated and wounds were observed by histologicalexamination (Hematoxylin and Eosin staining and Massion's staining)on the 3rd,7th,10th days and 3 weeks postsurgery; 2. the rabbit BMSCswere isolated and cultured and the biocompability of Gelatin/PCL andPCL were evaluated by cocultured with MSCs in vitro,the amount ofMSCs on these scaffolds were calculated by 2,4,6,8 days after coculture,cell cycles were measured by flow cytometry and the attachment andgrowth of MSCs were investigated by Scanning electron microscopy(SEM).Result 1. The wounds in experimental group1 healed better thanthose in group2 and Control group.The Gt/PCL composite nanofibrous sacffolds achieved a good adherence to the wounds,epidermal cells onGt/PCL proliferated and differentiated to form a new epidermis almostlike a natural skin. experimental group1 had less amount of effusion,lightened the degree of inflammation, decreased the proliferation ofgranulation tissue and shortened the healing period; 2. The primarygeneration MSCs can be isolated by panmeyloid method,MSCs wouldconflence after about 10 days'culture.The amount of MSCs on the Gt/PCL and PCL scaffolds slightly increased at the beginning of co- culture,and at the first 2 and 4 days the cells in control groups were far more thanthat in experimental groups,but at the following 6 and 8 days,the numberon Gt/PCL and PCL became obviously larger, and Gt/ PCL showed abetter capability of cell proliferation than PCL scaffold; 3. The flowcytometric analysis for 7 days'coculture exhibited that the index of cellproliferation was hightened,there was a statistical difference betweenGt/PCL and PCL(P＜0.05),and control grops(P＜0.05); 4. The SEMshowed that the BMSCs can attach and grow on the gelatin/PCL,and thecells spread well and migrate deep inside the gelatin/PCL scaffold.Conclusion 1. The Gelatin/PCL composite nanofibrous scaffold andPCL nanofibrous scaffold both obviously enhance the wound healing offull-skin injury; 2. The Gelatin/PCL composite nano-fibrous scaffold andPCL nanofibrous scaffold both show a good bio-compatibility with MSCs;3.The Gelatin/PCL composite nanofibrous scaffold is better than PCL nanofibrous scaffold.