| Objective:From ephedra soup, fruit powder, Mahuangtang tripartite co Xanthium Powder therapy on immune function in mice with chronic rhinitis cold type of start, explain show "ancient practice of" theoretical foundation to study the "ancient and modern standards side "of clinical significance to experimentally demonstrate" ancient practice of "scientific.Methods:60 mice in the tail mark, using the weight groups stratified random number table method, which is divided into control group, model group, Mahuangtang group, cocklebur wind scattered groups together Mahuangtang Cang ears scattered groups. Adaptive feeding week were chronic rhinitis cold type of modeling, a total of 21 days. SO2 exposure to excessive use of combined estrogen production intramuscular experimental model of chronic rhinitis, after modeling the end Mahuangtang group to ephedra decoction,17.369/kg, cocklebur Xanthium scattered to scattered groups decoction 17.369/kg, Mahuangtang co Xanthium Powder Group to Mahuangtang co Xanthium Powder decoction,17.369/kg, control group and model group with the same amount of normal saline. According to 0.2ml /lOg body weight administered in each group for consecutive 10 days after the last administration to eat, can not help but water drawn. First, blood samples were collected in order to properly detect the mouse CD4+T cells, CD8+T cell content and ratio, the concentration of IgA, the percentage of macrophages; nose of mice, thymus, spleen, lungs, and calculate the mice organ index, and then fixed in 10% formalin, paraffin-embedded after decalcification, which is made from biopsy, were mounted with HE staining, collecting various pathological images observed pathological changes in the organs, Electron microscopy thickness nasal mucosal epithelium calculate the number of goblet cells. SSPS17.0 with statistical analysis software for data processing.Results:The various organs of mice indices related immunological parameters were lower than the control group and the drug group, the difference was statistically significant (P<0.05), and (P<0.01); nasal morphology indicators higher than the control group and the drug group, the difference was statistically significant (P<0.05). Mahuangtang mice CD 4+T, CD8+T, IgA higher than the control group, the difference was statistically significant (P<0.01), and (P<0.05), spleen index, thymus index, final body weight of less than Fang group The difference was statistically significant (P<0.01); epithelial goblet cells is higher than the combined party group, the difference was statistically significant (P<0.01). Thymus Xanthium Powder Group, CD4+T, CD8+T, mucosal thickness lower than the control group, the difference was statistically significant (P<0.01); Fang group spleen index, thymus index and model group statistical difference significance (P<0.05), CD4+T/CD8+T cell ratio, respiratory IgA higher Xanthium Powder group, the difference was statistically significant (P<0.05), mucosal thickness, number of goblet cells in the mucosal epithelium lower than Mahuangtang group, macrophage phagocytosis higher than Mahuangtang group and Xanthium Powder group, the difference was statistically significant (P<0.01).Light microscopy cases show histological sections:the control group of normal mice nasal structure, no inflammatory changes. Model mice obvious nasal swelling, mucosal edema, and some are still swollen endothelial cells, submucosal connective tissue proliferation. Nasal tissue drug group were improved, but the co-party group of pathological changes better than the other two groups, the mouse lung tissue morphology was observed under the microscope control group of mice were normal lung tissue, alveolar congestion model mice, inflammatory cell infiltration, exudation, and accompanied by stromal hyperplasia, together party group in inflammatory response and morphological changes, the group was superior to ephedra soup, fruit powder group; control group of mice were normal thymus tissue structure, model mice thymocytes sparse arrangement, cortical thinning, medulla expanded corticomedullary unclear boundaries. Thymus cells arranged drug group compared with the previous close, thymic medulla widened, corticomedullary demarcation was clear, reticular epithelial cells decreased thymus visible bodies, suggesting the drug has improved its pathology, especially Fang significantly. The control group of mice spleen red pulp, white pulp clear boundaries, capsule intact. Splenic macrophages model group decreased lymphocyte count decreased, white pulp narrow, red pulp expand, accompanied by congestion, see follicles surrounding plasma cells. Fang group of white pith from the previous increase, lymphocyte count increased spleen pathological changes improve the performance of its pathology, with the model group than Mahuangtang group and Xanthium scattered groups.Conclusion:1. combined with excessive estrogen exposure SO2 intramuscularly, and with cold stimulation, the establishment of experimental animals can induce chronic rhinitis cold type model.2. ephedra soup, fruit powder on chronic rhinitis cold type mice with tissue morphology, immune organ index, immunology indicators have a positive impact, but Mahuangtang co Xanthium Powder comparison, the indicators below the Fang group may regulate immune function and related instructions Fang has certain advantages. From the point of view of animal experiments demonstrated that the "ancient practice round of the" scientific. |
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