The Use Of Cytokine Antibody Array Technology Study On Bidens Regulation Immune Inflammatorysignal Transduction Mechanism Of Dry Eye

Objective:To detect cytokine antibody array technology in this paper, combined with thedetection of ocular conjunctival tissue ultrastructure and inflammatory factors and apoptosis gene, clear the Bidens bipinnata by inflammation and apoptosis signal transduction pathwaywhich key cytokine treatment of dry eye syndrome, and to explore the mechanisms of Bidens pilosa L treatment of dry eye syndrome from the molecular level.Research contents:â‘ The establishment of two types of rat xerophthalmia model:inhibition of application of atropine eye drops of tear secretion secretion production lack of dry eye model tear, cauterization meibomian gland mouth closed production tear evaporative dry eye model. â‘¡ Before and after the rats, ultrastructural changes in tears and tear film changes and conjunctiva and lacrimal gland detected before and after treatment of Bidens bipinnata. â‘¢ Using immunohistochemistry (SP method) to observe the mold before and after, before and after the treatment of Bidens bipinnata conjunctiva and lacrimal gland tissue inflammatory cytokines (IL-1, TNF-alpha) and apoptosis genes (Bcl-2, Bax) expression.â‘£The application of cytokine antibody array (RayBioTM cytokine chip), each of the two types ofBidens bipinnata dry eye treatment group and saline control group rat tear with analysis on differential expression of cytokines, the derivation of Bidens bipinnata involved in immuneinflammatory signal transduction through which factors, to elucidate the mechanism of Bidens bipinnata L. multi targets for the treatment of dry eye in molecule the level of.Results:In statistical sense, dry eyes with atropine eyedrops rats model before and after its use the application from the perspective of the on the whole flow of tears and no significant difference (P> 0.05), and this difference mainly reflects in specific rupture time appears different (P<0.01); And by comparing the epithelial cells of TNF alpha as well as Fas as you can see significant changes, especially the Fas is obviously a lot less positive, and there is very big difference (P<0.01), IL is relatively less, and there is very big difference (P< 0.05). (2) in statistical sense, closed meibomian gland dry eyes model group rats tears flow contrast before and after treatment (P> 0.05), and this difference reflected in the specific rupture appears not much difference in time (P> 0.05). Closed the meibomian gland of rats under the condition of dry eye symptoms, compared before and after the no significant different (P> 0.05).Conclusion:For the treatment of dry eyes aspects, ghost needle grass has certain conditions, for its close the meibomian gland that does not produce effect. Currently only apply to the character of atropine eye drops. Ghost needle grass by beta, IL IL-1-4, IL-10, CINC-3, the difference of CNTF expression of signal transduction, can obviously improve the cytokine mediated by above of atropine eye tear secretion formed by lack of dry eyes, and to close the meibomian gland forming strong evaporation type dry eyes, only to the influence of IL-1 beta, the treatment effect is not obvious. Therefore believe that ghost needle grass by beta, IL IL-1-4, IL-10, CINC-3, CNTF, Fas Ligand transduction of cytokines, play to the role of the treatment of dry eyes, Fas Ligand in the atropine group guidance role in apoptosis pathway is greater than the inflammation factor signal channel; Closed meibomian gland set of lacrimal gland tissues IL-1 beta signal transduction plays a main role.