Research Of GPC3 And CD44v6 Expression In Hepatocellular Carcinoma

Objective: To investigate the expression of GPC3 and CD44v6 in hepatocellular carcinoma and understand whether GPC3 and CD44v6 are involved in the growth and development of hepatocellular function through synergistic signaling by Wnt pathway.Method: 1. 1. Immunohistochemistry was used to investigate the expression of GPC3 and CD44v6 in 38 HCC tissues.2. Results were analyzed through using SPSS for Windows 17.0 software analysis system. Chi-square test or Fisher’s exact test were employed for data processing,Spearman rank correlation was adopted for correlation analysis. P <0.05 was considered to have a significant difference.Results:GPC3 is mainly located in hepatocellular carcinoma tumor cell membrane and cytoplasm, with rare nucleus occasional expression; there was a significant difference(X2=3.924; P=0.02; X2=6.575; P =0.000; X2=6.862; P =0.000) between well-differentiated hepatocellular carcinoma group, differentiated group, poorly differentiated group and control group(cirrhosis group),; no difference between every two groups(X2=2.922; P=0.232). CD44v6 immunohistochemical staining substances were mainly localized in the cytoplasm of cancer cells and part of the tissue interstitium being diffused or scattered; when comparing well-differentiated HCC group,differentiated group, poorly differentiated group with the control group, there aresignificant differences in liver cirrhosis group(X2=4.486, P=0.001; X2=6.926, P=0.000;X2=6.194, P=0.000); no difference between every two groups(X2=3.571; P=0.168). At the 0.01 level(bilateral) GPC3 expression was significantly correlated with CD44v6 in hepatocellular carcinoma.Conclusions: The expression of GPC3 and CD44v6 in hepatocellular carcinoma was high, which has a significant correlation at the level of 0.01(bilateral).