The Effect Of Ieris Chinxensis On Nicotine Poisoning And Withdrawal In Mice

Nicotine is the main alkaloids in tobacco, which toxicity strongly. Nicotine is the maincause of nicotine dependence, which namely smoking addiction. Cigarette smokingcessation is a severe problem of mangy countries and our country. So the study of smokingcessation methods received widespread attention. This text research the antagonistic actionof Ieris chinxensis (IC) on nicotine acute and chronic poisoning and the effect of IC onnicotine withdrawal in animal tests. Provide the basis for further development of functionalquit smoking food. The main research contents and results are as follows:(1) Research the effect of IC on nicotine acute poisoning in mice and discover IChave antagonism effect on nicotine acute poisoning to some extent. Establish nicotine acutepoisoning model through subcutaneous injection nicotine solution. Acetylcholine contentwas taken as index to research the antagonistic effect of IC on nicotine acute poisoning bythe model. And contrast the effect of leave and root, as well as different extractive of dryIC and fresh IC. The results of this research show that,45min after given by gavage withIC samples, serum acetylcholine content markedly below model group（p <0.05）.Conclusion: IC could antagonism the acute poisoning of nicotine in mice. And the effectiveconstitutent exist in the leaves and root of IC. Vacuum freeze drying could save theeffective constituent of IC. The effective constituent could extract by60%ethanol solution.(2) Research the effect of IC on nicotine chronic poisoning in mice and discover IChave antagonism effect on nicotine chronic poisoning to some extent. Establish nicotinechronic poisoning model through subcutaneous injection nicotine solution. At the sametime, mice were given by gavage with IC sample for14d. The change of mouse bodyweight, distance travel in horizontal direction, rearing times, immobility times duringforced swimming, as well as the content of DA, GLU and GABA were taken as indexes toassess the antagonism effect of IC on nicotine chronic poisoning. The results of thisresearch show that, compared with negative control group, the distance travel in horizontaldirection, rearing times and immobility times during forced swimming of model grouphave a significant (p <0.01) increase, and have a significant (p <0.01) decrease on DA、GLU、GABA; compared with model group, IC group have a significant (p <0.01)reduction on the three behavioristics indexs, but have no difference to negative control group; compared with model group, IC group have a significant (p <0.01) increase on DAand GLU except GABA, but the three indexes have a significant (p <0.01) reduction tonegative control group. Conclusion: IC could restrain the change of neurotransmittercontent in brain and the withdrawal signs to some extent which be induced of nicotine, andhave antagonism effect on nicotine chronic poisoning to some extent.(3) Research the effect of IC on nicotine dependence withdrawal in mice and discoverIC could restrain the change of neurotransmitter content in brain to some extent which beinduced of nicotine withdrawal, and have obvious ameliorate effect on these nicotinewithdrawal signs. Establish nicotine withdrawal model through subcutaneous injectionnicotine solution for14d. The model animals were divided into3groups, ICⅠgroup, ICⅡgroup and model group. Another group was negative control group. ICⅠgroup was givenby gavage with IC after nicotine withdrawal. ICⅡ group was given by gavage with IC7dbefore nicotine withdrawal.14d after nicotine withdrawal, the change of mouse bodyweight, the distance travel in horizontal direction, rearing times, immobility times duringforced swimming, come out times from black case, as well as the content of5-HT、DA、NE、AD、ACH、GLU、GABA in brain were taken as indexes to assess the effect of IC onnicotine withdrawal. The results of this research show that, about14d after nicotinewithdrawal, compared with negative control group, the distance travel in horizontaldirection, and come out times from black case of model control group have a significant (p<0.01) decrease, and have a significant (p <0.01) increase on rearing times andimmobility times during forced swimming and weight gain, and have a significant (p <0.01) increase on the seven neurotransmitter content in brain, and nicotine withdrawalmodel was established successfully; compared with model control group, ICⅠgroup havea significant (p <0.01) increase on the distance travel in horizontal direction, and the DA、NE、ACH、GLU、GABA content in brain, have a significant decrease on rearing times (p<0.05) and weight gain (p <0.01); ICⅡ group have a significant increase on the distancetravel in horizontal direction,(p <0.01) and come out times from black case(p <0.05)andthe seven neurotransmitter content (p <0.01) in brain, have a significant decrease onimmobility times during forced swimming (p <0.05) and weight gain (p <0.01). In ICcontrol group, the four behavioristics indexs and the weight gain and the5-HT contenthave no difference to negative control group, but the other indexes are higher than thenegative control group. Conclusion: IC could restrain the change of neurotransmittercontent in brain to some extent which be induced of nicotine withdrawal, and have obviousameliorate effect on these nicotine withdrawal signs.