Effects Of Washed Autologous Blood Transfusion On The Erythrocyte Mechanical Damage In The Salvaged Blood From Diabetes

Objective:To explore the effect of abstergent autologous blood transfusion on the recovery and hemolysis of erythrocytes from diabetic patients subjected to off-pump coronary artery bypass grafting (OP-CABG).Methods:A total of Sixty patients were included in this study. The patients were divided into two groups:control (C, n=30), and diabetic group (D, n=30). Samples were taken preoperatively, prior to and after cell salvage processing to determine the recovery and fragility of erythrocytes. Free hemoglobin and extracellular potassium were measured at0,4,6,12,24h after washing. Results:The erythrocytic recovery did not have significant difference between two groups (P>0.05).Under the same processing, the erythrocyte fragility in the diabetic group were significantly higher than the control group in preoperation and prior to washing (P<0.05). However, after washing there was no significant difference of erythrocyte fragility between groups. Free hemoglobin and blood potassium at4,6,12,24h after washing were significantly increased (P<0.05) by a time-dependent manner in the two groups. But there was no obvious difference between groups at the same time point.Conclusion:Autotransfusion has no significant extra damage on erythrocytes in diabetic patitents undergoing OP-CABG, and the salvaged blood should be retransfused as soon as possible to reduce hemolysis.