Effects Of Malassezia On Secretion Of Cytokines On Seborrheic Dermatitis

Objective:(1)To Research the patients of seborrheic dermatitis in peripheral blood with interleukin-1β(IL-1β), interleukin-8(IL-8), interleukin-10(IL-10) and interferon-γ (INF-y) changes.(2)Malassezia with the HaCaT cells co-culture, study for HaCaT cells release interleukin1β (IL-1β), interleukin8(IL-8) and interleukin10(IL-10) and interferon-γ(INF-γ).Methods:(1)Screening seborrheic dermatitis patients, according to the rash of grouping the dominant strain of Malassezia, the ELISA method was detected in the patient group in peripheral blood of interleukin1β (IL-1β), interleukin8(IL-8), interleukin10(IL-10) and interferon-γ(INF-γ) change.(2) M.sympodialis and M.Globosa ratio of different concentrations, and in vitro human immortalized keratinocytes (HaCaT cells) co-cultured, methyl thiazolyl tetrazolium salt (MTT) method to determine the different Malassezia concentrations of HaCaT cell proliferation rate. By ELIS A at different time points (12h,24h,48h) culture supernatant of interleukin1β(IL-1β), interleukin-8(IL-8), interleukin-10(IL-10) and interference-γ (INF-γ) content.Results:(1)M.sympodialis and M.Globosa patients with seborrheic dermatitis detect serum cytokines compared with normal subjects the difference was not statistically significant.(2)The Malassezia and HaCat cells co-cultured, cells and fungal ratio1:20,1:30HaCaT cells proliferation activity by the M.sympodialis and M.Globosa significantly inhibit.(3)Cells and fungal culture12h,24h and48h, the the HaCat cells alone culture IL-1β secretion concentration3.57±0.59pg/ml,5.61±0.63pg/ml and10.60±1.66pg/ml;The M.Globosa with HaCaT cells co-cultured IL-1βsecretion concentrations were36.74±5.59pg/ml,59.08±5.09pg/ml and89.83±8.52pg/ml; the M.sympodialis with HaCaT cells co-cultured, IL-1β secretion concentrations were17.87±7.31pg/ml53.10±7.27pg/ml and85.67±9.08pg/ml.(4)Cells and fungal culture12h,24h and48h, the the HaCat cells alone culture IL-8secretion concentration3.83±1.02pg/ml,5.35±1.74pg/ml and6.45±2.23pg/ml; The M.Globosa with HaCaT cells co-cultured IL-8secretion concentrations were25.98±3.06pg/ml,43.64±4.37pg/ml and78.56±6.23pg/ml;the M.sympodialis with HaCaT cells co-cultured, IL-8secretion concentrations were17.87±7.31pg/ml,53.10±7.27pg/ml and85.67±9.08pg/ml.(5)Cells and fungal culture12h,24h and48h, the the HaCat cells alone culture IL-10secretion concentration2.45±0.41pg/ml,3.26±0.55pg/ml and6.77±1.83pg/ml; The M.Globosa with HaCaT cells co-cultured IL-10secretion concentrations were27.67±2.62pg/ml,41.68±8.56pg/ml and72.18±7.47pg/ml; the M.sympodialis with HaCaT cells co-cultured, IL-10secretion concentrations were32.10±3.66pg/ml,40.08±5.84pg/ml and67.88±7.01pg/ml.(6)Cells and fungal culture12h,24h and48h, the the HaCat cells alone culture INF-y secretion concentration Opg/ml, Opg/ml and0pg/ml;The M.Globosa with HaCaT cells co-cultured INF-y secretion concentrations were14.93±1.38pg/ml,29.99±3.59pg/ml and33.39±.64pg/ml;the M.sympodialis with HaCaT cells co-cultured, INF-y secretion concentrations were11.76±2.63pg/ml,28.83±2.45pg/ml and49.61±7.92pg/ml.Conclusion:(1)No significant increase of cytokines in serum of patients with seborrheic dermatitis than normal.(2)M.Globosa or M.sympodialis and HaCaT cells co-cultured with IL-1β,IL-8, IL-10and INF-y compared with HaCaT cells cultured alone increased; M.Globosa and M.sympodialis after co-culture with HaCat cells increased IL-10and INF-γ no obvious difference, but in M.Globosa and HaCat cells co-culture IL-1β and IL-8after the M.sympodialis cultivation increased more significantly with HaCat cells.