| Objective：In the understanding of relevant animal model under the premise of theexperimental data. Through the lavage liquor, and the methods of auxiliary long-termanger make rats alcoholic hepatitis, liver and kidney Yin deficiency syndrome animalmodel to copy success. Test to different doses of consistent among groups of rats aftermodified Yiguanjian serum biochemical indexes, and observed among groups in the ratliver pathological changes. Explore the effect of modified Yiguanjian on rats withalcoholic hepatitis of liver and kidney Yin deficiency syndrome of prevention and curefunction.Methods：1. Modified Yiguanjian preventing alcoholic hepatitis and liver and kidneyyin deficiency syndrome:40to200+10g only male SD rats.,feeding14days, make itsfully adapt to the environment.Randomly divided into5groups, every group of8.Including the control group, model group,modified Yiguanjian prevent low dose group,modified Yiguanjian prevention dose groups,modified Yiguanjian high dose group. Inaddition to the normal group,The rest every rat hind legs bound,make its anger,In angerafter1hour,control group according to their habits to give corresponding feed,alsoconforms to the proportion of distilled water to fill the stomach. Another groups to feed atthe same time, according to the weight of rats every day to give corresponding9g/kg doseof40degrees liquor to fill the stomach,until42days, In addition to the control group andmodel group, Another groups of rats after alcohol lavage1h, used in line with theproportion of concentrated Chinese medicine injection in rats in vivo. Model group inalcohol lavage after1h, use in line with the proportion of distilled water to fill thestomach.Adhere gavage42days.After the last gavage,the feed and water from the cageremoved about12hours.According to the proportion of the chloral hydrate is configured asa6%,according to the weight calculating charge. Anesthetized rats,blooding from theabdominal aorta, at the same time, divided the blood into two parts,part of the blood for thedetermination of estradiol (E2), testosterone (T) and liver function indexes;other part of theblood for the determination of cyclic adenosine monophosphate (cAMP),and the rat liverrepeatedly washed in saline solution,until there is no congestion.The liver of rats in everygroup respectively take the part of the organization to soak into pathological sectionsformalin fixed.2. Modified Yiguanjian in treatment of rats with alcoholic hepatitis and liver andkidney yin deficiency syndrome:24250±10g male SD rats were randomly divided intomodel successfully into3groups,8rats in every group,including modified Yiguanjian Treatment of low dose group, modified Yiguanjian treatment dose group, high dose groupof modified Yiguanjian treatment.The model group and the control group were given feedand water according to their characteristics,at the same time with distilled water inproportion by gavage.Another group were given feed at the same time, the use ofconcentrated Chinese herbs in proportion into rats,stick by gavage for42days.Lastgavage,the feed and water from the cage removed about12hours,according to theproportion of the chloral hydrate is configured as a6%.The blood from the abdominalaorta,and divided into two parts,Part of the blood for the determination of estradiol (E2),testosterone (T) and liver function indexes;other part of the blood for the determination ofcyclic adenosine monophosphate (cAMP).Liver repeatedly washed in physiological saline,until there is no congestion.The liver of rats in every group respectively take the part of theorganization to soak into pathological sections formalin fixed.Results：Modified Yiguanjian can due to the40degrees of liquor gavage with acertain degree of improvement by way of rats caused by diet decreased, poor spiritetc.Modified Yiguanjian on40degrees of liquor gavage caused by serum alanineaminotransferase (ALT), aspartate aminotransferase (AST), AST/ALT, total bilirubin(TBIL), gamma glutamyl transferase (γ-GT), alkaline phosphatase (AKP) increased acertain preventive effect.Modified Yiguanjian on anger caused by estradiol (E2), E2/T,cyclic adenosine monophosphate (cAMP) increased a certain preventive effect.ModifiedYiguanjian on total protein40degrees of liquor gavage induced (TP), albumin (Alb) haspreventive and therapeutic effect of reducing.Modified Yiguanjian on anger caused bytestosterone (T) have preventive and therapeutic effects of reduction.Modified Yiguanjianon rats with alcohol by gavage to have some role in prevention and treatment of liver cellinjury.And, modified Yiguanjian preventing alcoholic hepatitis and liver and kidney yindeficiency syndrome,the middle group and the high doses group have the betterprophylactic effect.Modified Yiguanjian in treatment of rats with alcoholic hepatitis andliver and kidney yin deficiency syndrome, the high dose group has the better treatmenteffect.Conclusion：Success made the rat model of alcoholic liver and kidney yin deficiencysyndrome.Modified Yiguanjian has a preventive effect on alcoholic liver and kidney yindeficiency syndrome.Modified Yiguanjian has a good therapeutic effect on liver andkidney yin deficiency syndrome in rats with alcoholic hepatitis. |
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