Study On Ginsenoside Rb1 Against Exercise Fatigue Induced Myocardial Apoptosis Of Calcium Overload And Ros Increased

Ginsenoside Rb1 is Panaxadiol Saponins monomer. The results show that the ginseng saponins delaying exercise-induced fatigue, and has the function of health care, but for ginsenoside Rb1 on sports fatigue induced cardiomyocyte apoptosis is inhibited, has not been reported。This paper mainly through rat swimming fatigue model was established, by intraperitoneal injection of ginsenoside Rb1, Rb1 is able to against exercise fatigue phenomenon of oxidative stress leads to apoptosis of myocardial cells were studied. Experiment is divided into four groups, control group respectively, movement fatigue group, low concentration treatment group(Rb1 5mg / kg) and Rb1 high concentration treatment group(Rb1 25 mg / kg). The application of(1) myocardial morphological changes: Hoechst staining detection found myocardial nuclei fatigue rats showed apoptosis obviously, after injection of Rb1 abnormal ratio decreased, and the protective effect of low concentration of Rb1 on myocardium was more obvious;(2) measurement of myocardial cell apoptosis rate of Tunel was found:exercise fatigue myocardial apoptosis rate increased, the apoptosis rate was significantly decreased after Rb1 injection, respectively:low concentration group(3.48 + 0.72%) and high concentration group(4.66 + 0.22%);(3) NADPH oxidase activity detection: Rb1 can reduce the activity of NADPH oxidase in fatigue rats, compared with the fatigue group, low concentration group Rb1 activity decreased 35.1%, the high concentration group Rb1 activity decreased by 24.8%;(4) the detection of myocardial cell Ros activity: fatigue exercise Ros causes oxidative stress, myocardial cell injury. Rb1 reduce ROS activity after treatment of low concentration Rb1, and fatigue group compared with ROS activity decreased by 25%, the RB1 high concentration treatment of rat ROS activity decreased by 18%.(5) determination of caspase-3 activity: sports fatigue activated caspase-3 activity, and the control group increased by 71%, decreased the activity of apoptosis protein after injection of Rb1 and the high concentration of Rb1 treatment in rats and fatigue group fell 22% compared to the content, after intraperitoneal injection of low concentration of Rb1 reduced by 20%.(6)calcium transients were measured: fatigue resulting in oxidative stress damage to the cell membrane system, resulting in intracellular calcium disorders, fatigue rat calcium transient amplitude increased by 12%, enhance myocardial contractility, but the transient fall time is prolonged, the muscle endoplasmic reticulum calcium pump intake of calcium content decreased and Ca2+- ATPase activity decreased; intraperitoneal injection of ginsenoside Rb1 can reduce the magnitude of the calcium transients, the process has been shortened, protect myocardial tissue, improve calcium pump intake of calcium, reduce the accumulation of calcium ions.Ginsenoside Rb1 can reduce exercise-induced fatigue in the process of oxidizing substances for energy and other oxidation reaction of endogenous reactive oxygen species Rosand reduce the accumulation of Ca2 + in myocardial cells,effectively reduce the levels of oxidative stress, while reducing the expression of caspase-3 protein, inhibit the apoptosis of myocardial cells;ginsenoside Rb1 by reduced NADPH oxidase activity of fatigue in rats and further reduce ROS, inhibit the apoptosis of myocardial cells of rats.And low concentration of Rb1(Rb1 5mg / kg) for sports fatigue induced oxidative stress leads to apoptosis of cardiac myocytes has better protective effect.