Analysis About Differential Expression Profiling And Target Gene Prediction Of UV-related MicroRNA In CD4~+T Cells From Systemic Lupus Erythematosus

Objective:To identify the differentially expressed UV-related miRNAs in CD4+ T cells from patients with SLE and normal human and predict their target genes and functions, we use gene chip technology to detect CD4+ T cells of peripheral blood of SLE patients and normal human with UV irradiation and without UV irradiation.Methods:1. Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral venous blood of SLE patients and healthy controls by density gradient centrifugation. CD4+ T cells were isolated using microbeads and protocols provided by the manufacturer and then UV irradiated.2. RNA was isolated with miRNA isolation kits.3. Detect the differences of miRNA expression in CD4+ T cells, which are from SLE patients and normal controls. One group is radiated by UVB with 24 hours, while another group is not.4. Using predictive software for target gene prediction and integrated results.Results:1. CD4+ T cells with UV radiation from SLE patients compared with CD4+ T cells with no radiation from SLE patients,28 miRNAs were up-regulated and 11 miRNAs were significantly down-regulated.2. CD4+ T cells with UV radiation from healthy controls compared with CD4+ T cells with no radiation from healthy controls,2 miRNAs were up-regulated and 3 miRNAs were significantly down-regulated.3. Target gene prediction and functional analysis shows the target gene of miR-423-5p、miR-630、miR-155 may be histone H3, H2B gene, and the target gene of miR-671-5p、miR-17、miR-222、miR-3156 may be MBD2 gene.Conclusion:The CD4+ T cells in SLE patients exist UV-related miRNA, which may play an important role in UV-induced lupus pathogenesis and aggravation. Histone H3, H2B may be the target gene of miR-423-5p、miR-630、miR-155,whereas MBD2 gene may be the target gene of miR-671-5p、miR-17、miR-222、miR-3156.