| Objective: To observe the mechanism of intestinal permeability changes in enterogenous infection through explored the relationship between the change intestinal permeability and intestinal flora shift when severe acute pancreatitis(SAP) occurring; To explore the mechanism of intestinal permeability changes induced by probiotics according to observe the effect of probiotics in the treatment to SAP. Methods:(1).54 SD rats, weight 250 ~ 250 g, male, were randomly divided into 3 groups; The sham-operation group(group A, n = 18), the SAP group(group B, n = 18) and the probiotic intervention group(group C, n = 18).(2).The sham-operation group were simplely treated with laparotomy, alonged by pancreas turning. While SAP and probiotic preparation groups were treated with 3% sodium taurocholic acid for preparation of SAP animal model, after Awake from anesthesia, group C were given a gavage with 10% probiotic preparations(bifidobacterium quadruple living bacterium piece, 1 ml/100g/times, Bid, lavage).(3)The experimental rats were anaesthetized at three time point(12 hours, 24 hours and 48 hours), 6 rats for each time point per group. We took venous blood 8 ml in inferior vena in Aseptic conditions, limulus reagent chromogenic azo matrix method is used to detect endotoxin, Determination of serum amylase level was carried out by automatic biochemical analyzer, enzyme mark double antibody sandwich ELISA method was adopted for the determination of TNF-a, IL- 6, serum D-lactic acid level.(4)we took the pancreas tissues for HE staining and used the kusske standard pathological grading to observe the damage degree of the pancreas; At about 10 cm far from ileocecal region, we cut out intestinal tube about 5 cm, axisymmetric cut open it along long axis, HE staining, Using improved Chiu’s score method to evaluate intestinal mucosal damage degree.The above data were collected with mean ± standard deviation(?x ± s),SPSS17.0 statistical software was used for Statistical analysis, The comparison of sample mean using single factor analysis of variance(one- way anova).Differences were considered significant at P﹤0.05.Results:(1)serum amylase level of group B(SAP) were higher than in group A(SO) group at all time points(P<0.05), the serum amylase level of Group C(probiotic preparations intervention group) were higher than group A(P﹤0.05) at 12 h and 24 h, but for 48 h, there is no statistical significance. The comparision of serum amylase level in group B and group C had no statistical difference(P > 0.05) at any time point.(2)endotoxin level of group B were higher than group A(P﹤0.05) at three time points. endotoxin level of Group C at 12 h, 24 h were higher than group A(P﹤0.05), but no statistical difference(P﹥0.05) at 48 h. for 24 h and 48 h, endotoxin levels of Group B were higher than group C at the same time point(P﹤0.05), no statistical difference was fond at 12 h(P﹥0.05).(3) the level of IL – 6 in group B and group C at 12 h, 24 h and 48 h were higher than in group A(P﹤0.05). level of IL – 6 in Group B at 12 h and 48 h were higher than group C(P﹤0.05), no statistical difference was found at 24 h(P﹥0.05).(4)TNF- a level of Group B and group C at 12 h, 24 h and 48 h were higher than group A(P < 0.05). TNF- a level of Group B at 12 h, 24 h were higher than C group(P < 0.05), no statistical difference at 48h(P > 0.05).(5)D- lactic acid level of Group B at 24 h and 48 h were higher than group A(P < 0.05), but no statistical difference was found at 12 h. D- lactic acid level of Group C at 24 h was higher than group A(P < 0.05), but for 12 h and 48 h,the comparisions were with no statistical difference. D- lactic acid levels of Group B at 24 h and 48 h was higher than group C(P < 0.05), no statistical differences at 12 h(P > 0.05).(6)pancreatic pathological grading of SAP group at 12 h, 24 h and 48 h were higher than control group(P﹤0.05), pathological score of group C at various time points were higher than group A than group B(P﹤ 0.05).(7)ileum tissue pathological score of SAP group at 12 h, 24 h and 48 h were higher than group SO and group C(P﹤0.05), Pathological score of group C was higher than group SO, but with no statistical significance(P ﹥0.05).Conclusion : 1.The Intestinal permeability increased in the early phase of severe acute pancreatitis, at 24 st hours to summit.2.The increased intestinal permeability is the main cause of enterogenic infection in severe acute pancreatitis.3.The improved intestinal permeability by a variety of mechanisms of Probiotics to reduce the enterogenic infection in severe acute pancreatitis. |
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