The Role Of Endotoxin And D-lactic Acid In The Early Diagnosis Of Severe Acute Pancreatitis With Intestinal Injury

Objectives The survey focuses on establishing severe acute pancreatitis animal model. Through dynamic testing the content of plasma endotoxin D-lactic acid,and observating the change of the pancreas, ileum pathological form, the author discusses the role of endotoxin and D - lactic acid in the early diagnosis of severe acute pancreatitis with intestinal injury.Methods 70 specific pathogen free (SPF) wistar male rats were divided into three groups randomly: control group (Control),Sham-operated group(Sham),and pancreatitis group.Sham and pancreatitis group are divided into 6 hours, 12 hours and 24 hours three time points.Pancreatitis was induced by injection of 5% Na-Tc under membrane of pancreas. The rats were killed in sixth hours'time after being operated in Sham-operated group. The rats were killed in the sixth, twelfth and twenty-fourth hour after Na-Tc injection in Six hours after pancreatitis group, Twelve hours after pancreatitis group and Twenty-four hours after pancreatitis group respectively. The content of amylase in serum was measured by holo-automatic biochemical analysator. Pancreatic tissues were observed by hematoxylin-eosin (HE) staining and counted for the pancreatic tissues and histological scores. At same time detected the concentration of Endotoxin ( ET) in plasma with ELISA method. Adopting enzymology spectrophotometry detection of serum D - lactic acid content .All the data were analyzed by statistics software SPSS17.0. Normal distribution measurement data shown as means±standard deviation ( x±s). One-way ANOVA method was applied to comparison among groups. And using S-N-K method for binary comparison. Pearson correlation analysis was applied to measure the relevant relations. The inspection standards was alpha = 0.05.Results We successfully established severe acute pancreatitis animal model. The rats of pancreatitis group exhibited a high amylase and severe pancreatic pathological damage after modeling.The ileum appears pathological damage after building mode 24h. There are non- significant difference (P>0.05) in sham group and control group and sham group each time in Pancreas pathological scorein;but in different periods,SAP groups was significantly different compared with sham-group and blank group;SAP group has significant differences compared with each other.The content of endotoxin and D-lactic acid in sham-operation group each time and blank group has no significant differenc(eP>0.05);but in different periods,SAP groups was significantly different compared with sham- group and blank group(P<0.05);SAP group has significant differences(P<0.05) compared with each other. The contend of SAP group of plasma endotoxin, D-lactic acid and pancreas pathological score exists linear correlation. This kind of correlation also exists between the content of plasma endotoxin and D-lactic acid.Conclusions We successfully established the severe acute pancreatitis models by using the method of injecting 5% Na-Tc under membrane of pancreas. The SAP intestinal damage of plasma endotoxin and D - lactic acid are increased in the early period, and gradually increased with the increase of pancreas pathological injury. Therefore,detection plasma endotoxin and D-lactic acid can be used as the current theoretical basis of early diagnosis in severe acute pancreatitis with bowel function injury.