The Effect Of Ehuang Decoction Retention Enema To Human Colorectal Carcinoma SW480 Xenograft Tumor EGFR Expression Level And Its Clinical Signification

Objection :To research the effect of Ehuang decoction retention enema to human colorectal carcinoma SW480 xenograft tumor EGFR expression lev-e l and its clinical signification.Methods :the SPF 2 BALB/c mice, female, 4-6W, G:16-22g; 50 SD rats,male and female, body weight(200 + 15) g was selected. The first preparation of model SPF level BALB/c solid tumor in mice with solid tumor, SDrats were given for the orthotopic transplantation tumor model, after the transplantation succed, select 40 successful rat model, according to the statistical random distribution principle, 40 rats were divided into 4 groups: group A to model control group, group B as positive model control group, group C as EHuang decoction high dose group, group D as EHuang Decoction low dose group, 10 rats in each group of SD rats. 2 weeks after inoculation. The drugs were given for treatment. The specific administration methods: group A enema with Sterile Water for Injection 10 ml / Kg,q d; group B intraperitoneal injection with 5-FU 50mg/kg, qod; group C,2m l(8G)/weight,enema,qd;group D,2ml(0.8g)/weight enema,qd.The 4 group was given the same normal diet and same feeding conditions and treated for 2 weeks. Objection is to observe the effect of Ehuang Decoc-tion retention enema on human colorectal carcinoma SW480 xenograft tumor growth and tumor inhibiting effect, and S-P immunohistochemical b was u-sed to analyze the expression of epidermal growth factor receptor in tum-or.Results:1, Ehuang Decoction retention enema in general conditions can improve SW480 transplanted tumor in rats;2, Ehuang Decoction inhibited SW480 transplanted tumor in rat tumor growth;3, Ehuang Decoction of each dose of tumor inhibition rates were 26.59%, 15.51%;4, Ehuang Decoction relative fluorouracil antitumor effect is weak;5, The effect of Ehuang Decoction retention enema and fluorouracil in traperitoneal injection in SW480 transplantation tumor expression of EGFR is similar, all of those can reduce the expression of EGFR.6, Ehuang Decoction inhibited the growth of tumor cells in rat SW480 xenograft tumor and promote the apoptosis of tumor cells.Conclusion: "Ehuang Decoction" retention enema on tumor cells of rat SW480 xenograft tumor growth has obvious inhibiting effect,.Ehuang decoction could improve the anti-tumor ability of the organism, reduce tumor E GFR expression level, restraints tumor cell growth and promotes tumor cell apoptosis.The experement provides powerful evidence and research reference for the further clinical application of Ehuang decoction.