Expression Of Cell Apoptosis Related Proteinin Hippocampus Of Rats With Autism And Electroacupuncture Changqiang Point Of Intervention Effect

Objective:1. The observation cell apoptosis related protein bax、bcl-2 in the VPA autism model the expression of the hippocampus of rats.2. The regulation on cell apoptosis related protein bax、bcl-2 in the hippocampus of VPA autism rats after electroacupuncture changqiang point.Methods One:Healthy adult Wistar male and female rats 1:1 cage, the second day early morning, Observe the tray in rats, if there is Yin chuan, is counted as embryonic day 1, pregnant female mice kept separately. Pregnant rats were randomly divided into two groups, one group at E12.5 (at 12.5 days) by intraperitoneal injection of the VPA (valproic acid sodium), birth of mice in model group; of another group of normal delivery, gave birth to the blank group. Rat after open time, damaging perception evaluation, selected autism in rats, to a Morris water maze test the ability of learning and memory. Random take blank group and model group rats all 12, only under the same environment raised 20 days, no intervention. End of the course of the second day in the water maze test again, break in the brain, observed by immunohistochemistry and western blot method in the rat hippocampus bax, bcl-2 protein expression.Methods Two:Healthy adult Wistar females and males 1:1 cage for the night, the second day morning, observed the females of Yinshuan for embryonic day 1 points kept separately in a cage. Pregnant rats were randomly divided into two groups, one group at E12.5 (at 12.5 days) by intraperitoneal injection of the VPA (valproic acid sodium), of birth for the model group; Another group of normal delivery, of birth for the blank group. Rat after open time, damaging perception evaluation, selected autism in rats, to a Morris water maze test the ability of learning and memory. Take 36 rats were randomly divided into 12 for"ChangQiang" electroacupuncture group ("ChangQiang" group),12 for non-acupoint electroacupuncture group (non-acupoint group) and rest 12 for non-acupuncture control group (model group), then randomly selected 12 for the blank group. "ChangQiang" group take "ChangQiang" point, including to continuous stimulation (continuous wave, frequency of 2 hz, strength of 2 mA) 20 min after the needle, continuous treatment of 20 d for a period of treatment, a total of 1 course of treatment;non-acupoint group to choose the horizontal edge of rats at the right side rib refers to, operation with "ChangQiang" group; The blank group and model group, under the same condition, will not be any intervention. End of the course of the second day in the water maze test again, break in the brain, observed by immunohistochemistry and western blot method in the rat hippocampus bax, bcl-2 protein expression.Results One:1.Open experiment:compared with the blank group, model group open late, PN12 indifference, PN13, PN14, PN15, PN16 have significant difference (P< 0.05).2.The pain caused by hot plate test, compared with the blank group, model group increased pain threshold, PN9, PN11, PN13 and PN15 have significant difference (P< 0.05).3. Morris water maze:before treatment, compared with the blank group, model group, on average, fewer prolonged latent, cross platform, there are significant differences (P< 0.05). After treatment:compared with the blank group, model group dropped the average latency time extended, cross platform and have significant difference (P< 0.05).4.hnmunohistochemical:Compared with the blank group, model group bax protein expression up the bcl-2 protein expression and lower, there are significant differences (P< 0.05).5.Western blot:Compared with the blank group, model group bax protein expression up the bcl-2 protein expression and lower, there are significant differences (P< 0.05).Results Two:1.Open experiment:with one open test results.2.The pain caused by hot plate test, with the results of a medium pain caused by hot plate test.3.Morris water maze:before treatment, with the result before one period of treatment. After treatment:compared with model group, the blank group, the average latency time shorten "ChangQiang" group, through the platform number increases, there are significant differences (P< 0.05), non-acupoint group, the average latency time shortened, and through the platform number increased, there was no statistically significant difference (P> 0.05).4.Immunohistochemical:compared with model group, "ChangQiang" group increased bax protein expression, the bcl-2 protein expression is reduced, there are significant differences (P< 0.05); The non-acupoint group increased bax protein expression, the bcl-2 protein expression decreased, there was no statistically significant difference (P> 0.05).5.Western blot:compared with model group, "ChangQiang" group increased bax protein expression, the bcl-2 protein expression is reduced, there are significant differences (P< 0.05); The non-acupoint group increased bax protein expression, the bcl-2 protein expression decreased, there was no statistically significant difference (P> 0.05).Conclusion:1.The cell apoptosis related protein bax expression decreased, increasing the bcl-2 protein expression in the hippocampus of the VPA autism rats.2.electroacupuncture Changqiang point can be suppressed by activating the hippoc-ampus bax, the expression of bcl-2, control the hippocampus of rats with autism bax, the proportion of the bcl-2 adjusted to abnormal development of the neurons, which have certaineffect to improve on their learning and memory ability.