Study On The Anti-aging Activity Of Tricholoma Lobayense Polysaccharides

Senescence is a universal phenomenon, and anti-aging is a popular project in scientific research. According to the free radical theory for aging, degenerative change in the aging process is mainly caused by the harmful effects of free radicals from cell metabolism. Accordingly, antioxidant is the first step to prevent he free radical damage. Therefore, finding a natural antioxidant medicine to chieve anti-aging effect by scavenging free radicals has become important.Tricholoma labayense Hiem is a kind of precious edible fungus in development. Based on the previous work, we found that the polysaccharide fration extracted from Tricholoma labayense owned strong scavenging ability on free radicals with IC50 values similar to Vc, which significantly inhibited erythrocyte hemolysis and MDA in vivo. Therefore, whether the polysaccharide TLH-3 possessed considerable anti-aging activity remains to be further studied. In this study, the anti-aging activity of TLH-3 was explored through the establishment of cellular and animal aging models.In this study, our group observed the protective effects of Tricholoma lobayense polysaccharides (TLH、TLH-2、TLH-3) on oxidation damage in aged mice induced by D-galactose. VE was taken as the positive group. 50 days latter, the samples were taken for detection, including the activities of SOD CAT and the levels of MDA in skin, serum, heart tissues.The results were as follows:(1) The activity of SOD, CAT in serum, liver, skin and heart tissue of D-gal group was significantly decreased than Control group;(2) Tricholoma lobayense polysaccharides groups could increase the activities of SOD、CAT and decreased the level of MDA compared with the aging model group;(3) The order of anti-aging effects of Tricholoma lobayense polysaccharides on anti-aging mouse is TLH-3>TLH>TLH-2. TLH-3 has the best anti-aging effects.Tricholoma lobayense polysaccharides can increase antioxidant enzyme activity, remove excessive oxygen free radicals, and reduce or stop the destruction of cells and tissues, thus protecting the normal function of cells and tissue and delaying the aging process induced by D-galactose.To further demonstrate the anti-aging effects of TLH-3, we build the model of cell senescence. Cell viability was measured by MTT assay, cell cycle and apoptosis analysis, by SOD activity and MDA detection of cell culture medium to determine the protective effects of TLH-3.The results were as follows:(1) The LC50 was observed at 600 μmol/1 concentration of t-BHP;(2) The cell viability increased in a dose dependent manner when the cells were pretreated with TLH-3;(3) Hoechst 33342 staining showed that, HELF cell nuclei were round and stained homogeneously in the control group. In t-BHP-treated group, a considerable proportion of cells displayed characteristics of apoptosis with condensed and fragmented nuclei. Treatment with TLH-3 led to a significant reduction in the number of apoptotic cells with fragmented nuclei.(4) TLH-3 could slow down G2 arrested that caused by t-BHP;(5) Apoptosis analysis showed that TLH-3 can significantly reduce the cell apoptosis rate, and improve the living ratio of cells compared with the untreated cells;(6) SA-β-galactosidase staining showed that,40% percents of cells are X-Gal dye masculine of aging group, under the intervention of TLH-3, the ratio decreased.(7) TLH-3 could promote damaged HELF cells to repair, improve SOD activity、 decrease MDA content and ROS level.Altogether, TLH-3 may reduce G1 phase arrest of HELF cells by increased the activity of SOD, decreased the level of MDA, ROS and then reduced the damage of HELF cells from oxidative.