Fabrication And Characterization Of A Novel Starch-based Haemostatic Material

This study sought to fabricate a novel haemostatic material by crosslinking starch-hyaluronic acid with sodium trimetaphosphate (STMP) using freeze-drying technique. And then its structure and properties are characterized. Finally, the haemostatic effect and biological compatibility are verified by animals experiment. The results showed that this novel material with three-dimensional network was crosslinked with STMP by FTIR and solid 31P NMR spectra. Hyaluronic acid could improve the water absorption and haemostatic ability in a large scale. The introduction of STMP could adjust the swelling behavior and thermal stability of materials to a certain extent. Overall hemostatic properties of materials was obviously better than that of Arista, gelatin sponge, and yunnan baiyao. Animal experiments have confirmed that average hemostasis time of the new hemostatic materials was 224.83 s, compared with Arista group of 271.83 s and blank group of 360.67 s. Thus we speculate that rapid hemostatic mechanism of the materials mainly depend on three-dimensional network, strong water absorption properties of hyaluronic acid and endogenous hemostasis. Animal experiments also demonstrate its good biological compatibility, namely no intradermal and sensitization reaction, no acute systemic toxicity, no cytotoxicity. To sum up, this novel starch-based hemostatic material is expected to be widely used in clinical applications.