Preparation And Biocompatibility Of Hyaluronic Acid Microspheres

Objective:To construct hyaluronic acid(HA)microspheres to assess the feasibility of using the microspheres as one of the tissue engineering seed cells-human pulp stem cell(Human dental pulp stem cells,hDPSCs)carriers to determine the effect of the microspheres on hDPSCs cell behavior.Methods:Hyaluronic acid microspheres were prepared by emulsification-cross-linking method.The gross appearance and microstructure of the microspheres were observed by naked eye and scanning electron microscope(scanning electron microscopy,SEM).The particle size and particle size distribution characteristics of hyaluronic acid microspheres were detected and analyzed by nano-size analyzer.The microspheres were co-cultured with hDPSCs,and the growth and proliferation of hDPSCs in the microspheres were evaluated by thiazole blue(methylthiazol tetrazolium,MTT)detection.The adhesion,stretch and growth of hDPSCs on hyaluronic acid microspheres were observed by using SEM.Results:Hyaluronic acid microspheres were successfully prepared.The microspheres were regular,smooth and round,uniform in size and particle size.The observation of cell adhesion experiment showed that hDPSCs could adhere to the surface of hyaluronic acid microspheres,the cells could fully stretch and grow well on the surface of the microspheres.MTT detection results showed that hyaluronic acid microspheres not only had good cytocompatibility,but also significantly promoted hDPSCs proliferation(compared with the microsphere-free group,p﹤0.05).Conclusion:Hyaluronic acid microspheres were successfully prepared.The microspheres are regularly smooth and round,uniform in size.The microspheres not only possess excellent cytocompatibility,but also can promote hDPSCs growth and proliferation.They might be applied as hDPSCs scaffolds for dental pulp tissue engineering.