| Purpose:To observed the expression of DCLK1 and Ki67 in normal mucosa, adjacent tissues, adenoma and adenocarcinoma; To analysis the significance of DCLK1 and Ki67 between the expression and clinicopathological parameters in colorectal cancer tissues; Focused on the distribution, morphological characteristics, quantity of DCLK1+/Ki67- cancer stem cells in solid tumors and human colon cancer cell LoVo, then, to explored the relationship between patients prognosis and clinicopathological parameters in adenocarcinoma, and to determined the specific immune phenotype and biological characteristics of colorectal cancer stem cell, as a new target for anti-tumor therapy.Method:To selected 150 cases of colorectal cancer tissues,34 cases of adenoma,10 cases of normal mucosa tissue and adjacent cancer tissue handmade tissue microarray. To observed the cell morphology, arrangement, distribution location of different types tissue and LoVo cells by using HE staining. Immunohistochemical staining showed the expression of DCLKl and Ki67 in normal mucosa, adjacent tissues, adenoma and adenocarcinoma tissues. To compared the cell morphological characteristics of DCLK1 and Ki67 with HE staining of adjacent section by using immunohistochemical double labeling in adenocarcinoma. Immunohistochemical staining and double staining showed the distribution, morphological characteristics, number of DCLK1+/Ki67-cancer stem cells in human colon cancer cell LoVo. By using statistical software SPSS19.0, to analysised the significance with the expression and clinicopathological parameters of DCLK1, Ki67 and DCLK1+/Ki67- cells in colorectal cancer LoVo cells.Result:1. HE staining of normal intestinal epithelial tissue was arrangement ruled, was glandular during which can be seen in goblet cells, columnar glandular cells, was abundant cytoplasm, nucleus was located at the base, none had atypia and pathological mitotic; adenoma can be seen that epithelial proliferated pseudostratified and nuclei was elongated rod in dysplasia tissue, some cells had atypia; cell disorder was observed in adenocarcinoma, had irregular glandular or diffuse distribution, large nuclei and deep stained, and atypia, pathological mitotic figures can be seen.2. The differences of Ki67 and DCLK1 in adjacent normal mucosa and carcinoma and adenoma tissues and cancer tissues was significant(P<0.05); and in the adjacent normal mucosa and adenoma and carcinoma tissues was not significantly different (P> 0.05).3. The expression of DCLK1 in colorectal cancer was related to location, depth of invasion, lymph node metastasis (P<0.05), but that of the histological type, gender, age was not statistically significant (P> 0.05). The expression of Ki67 was related to depth of invasion, histological type in cancer tissue (P<0.05), but that of gender, age, lymph node metastasis was not statistically significant (P> 0.05).4. DCLK1+/Ki67’cells were mainly distributed in the base of the crypt of the intestinal mucosa and in common wall of gland. DCLK1/Ki67* cancer cells was ovoid, and nuclei was deep stained and round or oval, prominent nucleoli, rare mitotic, less cytoplasm.5. In colorectal cancer tissues the expression of DCLK1+/Ki67’cells was related to histological type, depth of invasion and lymph node metastasis(P<0.05). Followed up for survival in patients was found that with high expression of DCLK1+/Ki67" cells were significantly lower than those with low expression (P<0.05), and metastasis and recurrence rates were higher than those with low expression (P<0.05), and the more increased in expression, the more increased in the rates of metastasis and recurrence and mortality.6. The number of DCLK1+/Ki67" cells accounted for 4.43% in colorectal cancer tissues, and for 6.59%in human colon cancer cell LoVo, mere was no statistically significant between them (P> 0.05).Conclusion:1. DCLK1 would help us understand the relationship between the occurrence, development and metastasis of tumors.2. DCLK1+/Ki67" can be as a cancer stem cell markers in colorectal cancer.3. The number of DCLK1+/Ki67- cells was significantly associated with the prognosis of patients in colorectal cancer, its can be used as pathological prognostic evaluation index. |
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