Effects Of Golph3 Associated With The Development Of Gastric Cancer By MTOR Signaling Pathway

Objective:To explore the clinical significance and mechanisms of GOLPH3 associated with the progression of gastric cancer by detecting the mRNA and protein expression levels GOLPH3 (Golgi phosphoprotein 3) and the major molecules of mTOR (mammalian target of rapamycin) signaling pathway in gastric cancer tissues.Methods:80 patients’gastric cancer, carcinoma-adjacent and paired normal tissues were collected. The mRNA and protein expression levels of GOLPH3 and the major molecules of mTOR signaling pathway were detected using RT-PCR, western blotting and immunohistochemistry. The relationship among the molecular markers and clinicopathologic factors was analyzed.Results:GOLPH3, p-AKT, p-mTOR, p-p70S6 and p-4E-BPl were expressed in gastric cancer tissues, which were associated with histological grade, lymph node involvement, depth of invasion and distant metastasis (p< 0.05) but not relevant to age and gender (p>0.05). The mRNA expression levels of GOLPH3 and the major molecules of mTOR signaling pathway in gastric cancer tissues were significantly higher compared with carcinoma-adjacent and paired normal tissues (p<0.05). The protein expression levels of GOLPH3 and the major phosphorylated protein of mTOR signaling pathway in gastric cancer tissues were significantly higher compared with carcinoma-adjacent and paired normal tissues (p<0.05). A strong positive correlation was observed between GOLPH3, p-mTOR, p-p70S6 and p-4EBP1 expression (r=0.410,0.303 and 0.276, respectively, p<0.05), but no significant correlation between the expression of GOLPH3 and p-Akt was observed (p>0.05).Conclusions:There is a phenomenon that GOLPH3 and AKT/mTOR signaling pathway were activated in human gastric cancer. GOLPH3 may affect the occurrence and development of gastric cancer by AKT/mTOR signaling pathway.