Diagnostic Value Of Golgi Phosphoprotein 3 In Primary Hepatocellular Carcinoma

Objective To explore the diagnostic value of serum GOLPH3 in primary hepatocellular carcinoma(HCC).To explore the relationship between the expression level of GOLPH3 in serum of HCC patients and clinicopathology.Methods From July 1,2016 to December 30,2017,We selected 70 patients with liver cancer from the Second Affiliated Hospital of Fujian Medical University and the First Affiliated Hospital of Wenzhou Medical University.17 patients with metastatic liver cancer and 4 patients without treatment were excluded.The remaining 49 cases of HCC(31 were treated with radical operation and 18 cases with interventional therapy).55 healthy volunteers(excluding liver space-occupying lesions by Ultrasonography examination)were included in control group.The expression levels of serum AFP and GOLPH3 in patients with hepatocellular carcinoma,patients with metastatic liver cancer and healthy control group were detected by electrochemiluminescence(ECL)and ELISA.The diagnostic value of serum GOLPH3 level in the HCC and the relationship between the expression level of GOLPH3 in serum of HCC patients and clinicopathology were analyzed by operating characteristic curve(ROC curve).SPSS21.0 was used for data processing.The difference was statistically significant when P <0.05.Results 1.The serum GOLPH3 level in HCC group,metastatic liver cancer group and healthy control group were 23.2067±5.2868ng/L,9.7129±4.78759ng/L and 3.5698±1.6393ng/L,respectively.The serum GOLPH3 levels in HCC patients were significantly higher than those in metastatic liver cancer patients and healthy controls(P <0.05).2.The serum GOLPH3 levels in HCC patients were not statistically different in different age groups,gender,smoking history,drinking history,hepatitis B prevalence,tumor diameter,tumor number,differentiation,microvascular thrombosis,liver function grading,and clinical staging of Chinese liver cancer(P >0.05),but statistically different in the prevalence of hepatitis B(P <0.05).3.AFP(alpha-fetoprotein)is the most important serological marker in the diagnosis of HCC;The sensitivity of differential diagnosis of HCC by GOLPH3 and AFP was 98.0% and 69.40% respectively,and the specificity was 96.3% and 97.6%,respectively.Analyze the differences between the two indicators then plot the ROC curve for diagnosis of HCC.The area under the ROC curve of GOLPH3 and AFP for diagnosis of HCC was 0.989,0.806,respectively.4.The combined sensitivity of GOLPH3 and AFP is 100%,The sensitivity of AFP and GOLPH3 in the diagnosis of HCC is significantly higher than that of AFP and GOLPH3.5.The serum GOLPH3 level in HCC patients within 3 days after treatment was not significantly lower than that before treatment(P > 0.05).After 4 weeks of treatment,the serum GOLPH3 level of HCC patients was significantly lower than that of preoperation(P < 0.05).6.AFP,GOLPH3,clinical staging and treatment of liver cancer in China are risk factors in the prognosis analysis Among the factors affecting prognosis of HCC,and the difference is statistically significant(P < 0.05).Conclusion 1.The expression level of serum GOLPH3 can be used in the auxiliary diagnosis of HCC patients.2.The combined diagnosis of serum GOLPH3 and AFP can effectively improve the diagnostic efficacy of HCC.3.Serum GOLPH3 levels in patients with primary hepatocellular carcinoma and different age groups,gender,smoking history,drinking history,tumor diameter,tumor number,differentiation,microvascular thrombosis,liver function classification,liver cancer clinical staging no obvious connection,Serum GOLPH3 levels positively correlated with HCC hepatitis B.4.The expression of GOLPH3 in serum has certain value in evaluating the curative effect of primary hepatocyte operation,following up after operation and predicting prognosis.