The Effect Of Peng-hui On Glucose-metabolism In Diabetic Rats

Objectives The model of diabetic rats was established by high fat and sugar diet feeding combinedwith the intravenous injection of a small dose of injection of streptozotocin (Streptozocin, STZ).The studyis to investigate mechanism of Peng-hui effect on model of diabetes rats on glucose metabolism, tumornecrosis factor-α (TNF-α), pancreatic tissue inflammatory cell count, pancreas and skeletal muscleexpression of IRS-1and other indicators, to explore its impact on blood sugar fluctuations part mechanismfor the prevention and treatment of diabetes may provide a basis.Methods Male SD rats were fed with high fat and sugar after8weeks, intraperitoneal injection ofsmall doses of STZ (35mg/kg),1week measured fasting blood glucose, blood glucose≥16.7mmol/Lfor the successful model rats54, into a mold after the rats were randomly divided into diabetic controlgroup, Peng-hui low dose group, middle dose group and high dose group. Low, medium and high dosegroup were given orally50,100,150mg/kg Peng-hui, and normal control group. Normal control groupand diabetic control group given normal saline orally per day, a total of8weeks. During the feeding,fasting blood glucose monitoring (FBG), fasting weight. At8weeks, the rats were sacrificed, serumfasting blood glucose (FBG), fasting insulin (FINS), C-peptide, tumor necrosis factor-α (TNF-α) changes.Insulin resistance index (IRI). HE staining of pancreatic tissue taken to calculate inflammatory cell counts.Pancreas and hindlimb quadriceps tissue, immunohistochemistry was used to detect rat pancreas andskeletal muscle IRS-1expression.Results The levels of blood glucose,,IRI of the diabetic rats fed with high-fat-high-sugar andjnjected with a small dose STZ were markely increased as compared with those of the normal control group.Insulin, C-peptide of the diabetic rats was reduced as compared with that of the normal control group.Those accorded with the feature of diabetic pathogenesis and biochemistry. Diabetic control group, low, medium and high dose Peng-hui rats compared with normal fasting blood glucose was significantly higherthan the control group, there was statistically significant (*P <0.05); diabetic control group, low, mediumand high dose groups Peng-hui fasting body weight, fasting insulin, C-peptide was significantly lowerthan the normal control group, there was statistically significant (*P <0.05); medium and high dosePeng-hui rat insulin resistance index than normal, diabetic control group were significantly increased, therewas statistically significant (*P <0.05); tumor necrosis factor-α (TNF-α) were not statistically differentbetween the groups (P>0.05). Diabetic control group, low, medium and high dose Peng-hui rats comparedwith normal control group, pancreatic tissue inflammatory cell counts were increased, there wasstatistically significant (*P <0.05). Peng-hui high-dose group than the control group, diabetic pancreatictissue inflammatory cell count increased, with statistical significance (△P <0.05). Peng-hui high-dose ratspancreas and skeletal muscle tissue expression of IRS-1levels were lower than the diabetic control group(*P <0.05) under the light microscope, diabetes control, low, medium and high dose Peng-hui ratspancreatic islet cells decreased, atrophy, acinar tissue vascular dilatation and congestion, peripheralinflammatory cell infiltration; skeletal muscle cells part myofibrils disorganized, visible crest dissolvedfracture.Conclusion1.Peng-hui diabetic male SD rats had no effect on blood sugar may, insulin resistanceindex was significantly increased.2.Peng-hui can cause diabetic SD rat pancreatic acinar tissue vasculardilatation and congestion, peripheral inflammatory cell infiltration, may aggravate diabetic SD rats,chronic low-grade inflammation.3. Peng-hui can reduce type2diabetic rat pancreas and skeletal muscleIRS-1expression levels and further aggravate insulin resistance.