Effect Of Tanshinone â…¡A On Cell Proliferation And Apoptosis Of Rectal Carcinoma SW480

Objective:To observe the effect of tanshinone Ⅱ A (Tanshinone Ⅱ A) on humancolorectal cancer cells SW cells proliferation and apoptosis of vascular endothelialgrowth factor (vascular endothelial growth factor, VEGF) Expression, to explore themechanisms of its anti tumor.Methods: With different concentrations of Tan Ⅱ A (final concentration of0.5,1,2,5,10.0ug/ml) treating SW480cells, the cell proliferation was tested by MTTassay; Detection of apoptosis of SW480cells by Annexin V-FITC ApoptosisDetection Kit; To detect the expression of SW480in mTOR cellsby SABC method; the expression of Real-time PCR detected SW480cellVEGF mRNA.Results:1、Detect Cell proliferation by MTT colorimetry: tanshinone IIa inhibitoryeffect on the proliferation of SW480cells showed a dose and time dependentmanner, with the increase of tanshinone IIa concentration, the survival rate of SW480cells was gradually reduced, and tanshinone IIa in the same concentrationof the inhibitory effect of prolonged SW480cell growthrate also increased.2、 Detect SW480cell apoptosis by Annexin V FITC method: Aftertanshinone IIa effects SW480for24h cells,SW480cells appeared apoptosisphenomenon, with the increase of concentrations of tanshinone IIa,SW480cellsshowed apoptosis and late apoptosis early increase proportionally. SW480cells showed apoptosis and late apoptosis early increase in the proportion of.3、Detect SW480cell expression mTOR by SABC method： SW480cell mTOR protein was mainly nuclear expression, and tanshinone IIa may inhibitthe expression of SW480cell mTOR protein.4、 Detect the expression of SW480cell VEGF mRNA byReal-time PCR： Expression of tanshinone Ⅱ A mass concentration of VEGFprotein were significantly decreased in all group.Conclusion:1Tanshinone Ⅱ A can inhibit SW480cell in vitro, and promote cellapoptosis.2Tanshinone Ⅱ A treatment of SW480cells resulted in downregulation of the expression of mTOR and VEGF.