Expression And Significance Of C-Rel In Actinic Keratosis,Bowen Disease And Cutaneous Squamous Cell Carcinoma

[Objective] To study the expression and significance of C-Rel in nomal skin,Actinic Keratosis, Bowen Disease and Cutaneous Squamous Cell Carcinoma.[Methods] The patients from who diagnosed standards as pathologically in dermatology department and pathology department Actinic Keratosis30cases, Bowen Disease30cases, Squamous Cell Carcinoma90cases, meanwhile, we also collected normal skin10cases from operating room of our department and dent facial surgery. After steps of dehydration, embedding, slicing and staining with immunohistochemistry (SP Method), observing slices under the microscope, each slice in the skin diseased tissue randomly selected five discontinuous visions; take a picture with OLYMPUS BX51Microscopic Image Acquisition System. Quantitative determination of each field of vision of positive expression of the average optical density with Image-Pro Plus6.0(IPP) Image analysis software.[Results]1.C-Rel in10cases of normal skin,30cases of Actinic Keratosis,30cases of Bowen Disease,90cases of well, moderately and poorly differentiated squamous cell carcinoma of the skin in all have positive expression. Normal skin, C-Rel is mainly expressed in epidermal basal layer cells and basal layer cells of the nucleus, the dye from basal layer to surface density and intensity gradually weakened, around the hair follicles, sweat glands and sebaceous glands of basaloid cells visible positive expression; Actinic Keratosis, C-Rel mainly expressed in epidermal basal layer and stratum spinosum lower1/3in the cell nucleus, dyeing density and strength, in the middle of stratum spinosum positive expression is scattered, the dermal papilla layer around a few positive lymphocytes expression; Bowen Disease, C-Rel is mainly expressed in skin full-thickness atypical hyperplasia cutin cell nucleus formation, the dermal papilla layer around a few positive lymphocytes expression,Well differentiated squamous cell carcinoma, C-Rel main expression is diffuse part around the nests of basaloid squamous cell nucleus of cells and nest, around the nests around a few positive lymphocytes expression; Moderately differentiated squamous cell carcinoma, C-Rel mainly is diffuse expression in squamous cell carcinoma of the nest, around the nests around a few positive lymphocytes expression;Poorly differentiated squamous cell carcinoma, C-Rel mainly diffuse expression in squamous cell carcinoma in the nest, around the nests around a few positive lymphocytes expression.2.By IPP software statistics, C-Rel expression as follows:the average optical density in normal skin is0.0045±0.0021; the average optical density in Actinic Keratosis is0.0239±0.0202, the average optical density in Bowen Disease is0.0510±0.0162; the average optical density in well differentiated Squamous Cell Carcinoma is0.0798±0.0188; the average optical density in moderately differentiated Squamous Cell Carcinoma is0.0823±0.0189, the average optical density in poorly differentiated Squamous Cell Carcinoma is0.0846±0.096.3.Statistical analysis, C-Rel in the expression of the average optical density between categories is different or not all the same (P<0.05), the difference was statistically significant. Further pairwise comparison, the normal skin group and well differentiated squamous cell carcinoma of the skin, normal skin group and moderately differentiated squamous cell carcinoma group, normal skin group and poorly differentiated squamous cell carcinoma of the skin, Actinic Keratosis and well differentiated squamous cell carcinoma group, Actinic Keratosis group and moderately differentiated squamous cell carcinoma group, Actinic Keratosis group and poorly differentiated squamous cell carcinoma, P is less than0.05, differences were statistically significant. The rest two comparison between groups (P>0.05), there were no statistically significant difference. Among them, the C-Rel strongest in the expression of poorly differentiated squamous cell carcinoma group, second is moderately differentiated squamous cell carcinoma group respectively, well squamous differentiated cell carcinoma group, Bowen Disease group, the Actinic Keratosis group and normal skin group. 4. C-Rel and expressed in each of the groups in the patients with different gender, different age, area of exposure and the exposure area were no statistical difference (P>0.05).[Conclusion]1.C-Rel and stratified squamous epithelial cell differentiation and proliferation of related;2.C-Rel in Actinic Keratosis and differential diagnosis value in the diagnosis of squamous cell carcinoma, both can be considered as a differential diagnosis of immunological indexes, but no obvious significance for squamous cell carcinoma pathological grading;3.C-Rel can serve as a epithelial tumor marker;4. Positive expression rate of C-Rel with different gender, different age, area of exposure and the exposure area were no statistical differences.