| Objective:this paper aims to test the expression of serum Hepcidin, related inflammatory cytokines, correlation index of liver function, correlation index of iron metabolism, correlation index bf anemia, liver Pro-hepcidin and duodenum Ferroportin of the chronic viral hepatitis B, C patients, so as to research the interaction mechanism between the inflammatory reaction and iron metabolism regulation as well as the anemia mechanism of chronic hepatitis B and chronic hepatitis C patients.-Methods:1. respectively select45cases of chronic hepatitis B and chronic hepatitis C patients,90patients with normal physical examinations in total. Divide these patients into chronic hepatitis B patients (HBV group), chronic hepatitis C patients (HCV group) and control group. Test serum Hepcidin, IL-1, IL-6, TNF-a, and CyPA by means of ELISA, test blood liver function index (ALT, AST), correlation index of iron metabolism (Fe, UIBC, TF, Ft), correlation index of anemia (RBC, Hb), HBV-DNA, HCV-RNA, and HCV antibody of chronic hepatitis B, chronic hepatitis C patients and healthy adults by means of professional instruments. And then statistically analyze the results.2. Select15cases of liver and duodenum specimens of chronic viral hepatitis C patients and12cases of liver and duodenum specimens of chronic viral hepatitis B patients, as well as12cases of liver and duodenum specimens from the control group, which are free of HBV, HCV, duodenum diseases and whole-body iron metabolic disease. Conduct semi-quantitative immunohistochemical analysis on Pro-hepcidin protein expression on liver specimens of HBV, HCV patients and patients of control group, and Ferroportin protein expression on liver specimens of duodenum by immunohistochemical method. And then statistically analyze the results.Results:1. the serum Hepcidin level of the HBV, HCV patients is obviously lower than that of the control group, presenting negative correlation with the serum Fe level, and the differences are of statistical significance (P<0.05). Serum IL-1, serum IL-6, TNF-a, CyPA level are all higher than that of the control group, and the differences are of statistical significance (P<0.05).2. It presents positive correlation (P<0.05) between serum Hepcidin and IL-1of the HBV patients, and presents negative correlation (P<0.05) between serum Hepcidin and CyPA, IL-6and TNF-a. It presents obviously negative correlation between the serum Hepcidin and IL-6of HCV patients, and presents positive correlation (P<0.05) between serum Hepcidin and TNF-a, CyPA.3. It presents obviously positive correlation (P<0.05) between CyPA and related inflammatory factor (IL-1, IL-6, TNF-a) in HBV and HCV patients.4. Serum Fe, Tf, TIBC and SF of the HBV patients are all higher than that of the control group, and the differences are of statistical significance (P<0.05). Serum Fe, Tf and SF of the HCV patients are all higher than that of the control group, but the serum TIBC is lower than that of the control group, and the differences are of statistical significance (P<0.05).5. It presents negative correlation between Hb and serum Fe and SF level (P<0.05).6. It presents positive correlation between serum CyPA and AST, ALT of the HBV patients (P<0.05).7. Liver Pro-hepcidin expression of the HBV and HCV patients are significantly reduced compared with the control group (P<0.05).8. Ferroportin expression of duodenum of the HBV and HCV patients are significantly increased compared with the control group (P<0.05).9. It presents negative correlation between the Ferroportin expression of duodenum of the HBV and HCV group and Pro-hepcidin expression of liver and serum hepcidin (P<0.05).10. It presents negative correlation between the Pro-hepcidin expression of liver of the HBV and HCV group and the serum Fe (P<0.05), and presents positive correlation between Ferroportin expression of duodenum and serum Fe (P<0.05).Conclusion:1. serum Hepcidin level of the HBV and HCV patients is obviously lower than that of the control group, and presents negative correlation between Hepcidin and serum Fe level. The decreasing in hepcidin level of HBV and HCV patients caused rising of the serum iron level.2. Regulation of inflammatory factor on Hepcidin in HBV and HCV is completed by different methods.3. CyPA may directly participate in the occurrence and development of the liver injury in the course of HBV of the HBV patients, and may promote the development of liver injury by cooperating with inflammatory cytokines. However, for HCV patients, CyPA can only indirectly participate in the occurrence and development of the liver injury in the course of HCV by means of inflammatory factor.4. In HBV and HCV, lowering Hepcidin level and increasing Ferroportin expression may cause increasing of the serum iron. |
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