Studies On The Relativity Between MMP-7Genetic Polymorphisms And Susceptibility Of Colorectal Cancer

Objective：To investigate the association of MMP-7gene-181A/G polymorphism andsusceptibility of colorectal cancer, MMP-7genotypes with colorectal cancer tumormarkers, the correlation between clinical pathological parameters. In the genetic andmolecular mechanism provides the basis.Methods：Randomly selected from2012to2013, the first affiliated hospital of Nan Changuniversity tissue samples of64patients with colorectal cancer confirmed surgery andperipheral venous blood of59cases of healthy controls， Using classical phenolchloroform one isoamyl alcohol to extract genomic DNA, the polymerase chainreaction-restriction fragment length polymorphism (polymerase chain reaction-restriction fragment length polymorphism; PCR-RLFP）method to detect MMP-7polymorphism, agarose gel electrophoresis to determine the MMP-7-181A/G sitegenotype.Detailed collection of colorectal cancer patients with preoperative diagnosisof tumor markers (CEA, CA19-9, CA12-5) and clinical pathological parameters.Results:(1) The healthy control group MMP-7sites-181A/G genotype frequency andallele frequency were accord with Hardy-Weinberg law of balance (χ2=1.91, P=0.166); MMP-7-181A/G site genotype frequency in case-control group had nosignificant difference of distribution, MMP-7-181A/G site genotype is not associatedwith colorectal cancer susceptibility (χ2=3.46, P=3.46, OR=0.49,95%CI=0.24-1.04); There was no significant difference in case group and healthy group ofMMP-7-181A/G sites A and G allele frequency distribution(χ2=2.62, P=0.106, OR=0.59,95%CI=0.31-1.12).(2) The tumor markers in patients with colorectal cancerand MMP-7genotype analysis: There was no significant difference inMMP-7-181A/G sites AA, AG+GG genotype and CEA、CA19-9、CA12-5value(respectiively P value was0.126、0.552、0.51）)（;3）In patients with colorectal cancer pathological parameters and MMP-7genotype analysis: there was no significantdifference in MMP-7-181A/G site AA, AG+GG genotype in the tumor site (colon,rectum)、different tumor gross type (ulcer type, uplift type)、different tumor size (≥5cm，<5cm)(respectively P value was0.679、0.147、0.071); There are significantdifferences in MMP-7-181A/G site AA, AG+GG genotype in tumor differentiationdegree (high differentiation+high-middle differentiation, low differentiation)、tumor infiltration depth (not to the full thickness,full thickness)、 lymph nodemetastasis(no transfer, transfer) and TNM staging in patients with colorectal cancer(stage III+IV, I+II)(respectively P value was0.045、0.037、0.001、0.000).Conclusion:(1) The MMP-7loci gene-181A/G polymorphism may be not associated withcolorectal cancer susceptibilit.(2) The MMP-7loci gene-181A/G polymorphism wasnot associated with colorectal cancer in clinical of tumor markers (CEA, CAl9-9,CA12-5).(3) The MMP-7loci gene-181A/G polymorphism was not associated withcolorectal cancer parts、general type and tumor size; But it was associated withcolorectal cancer differentiation degree、infiltration depth、lymph node metastasis andTNM stages, carry the G allele may promote the progress of tumor in patients withcolorectal cancer. Thus, carrying G allele palys a role in development of tumor and,MMP7gene polymorphism may be a factor during the progression of colorectalcancer.