| Objective:1: To detect the expression of pre-operative serum level of CEA,CA19-9 and LN in colorectal cancer patients.2: To analyze the relationship between blood CEA,CA19-9 and LN expression and clinic pathologic factors.3: To investigate the prognosis factors of the colorectal cancer, especially to identify the expression of CEA,CA19-9 and LN with different prognosis outcomes in colorectal cancer patients, as well as to explore the expression of three tumor biomarkers'affection related to the prognosis of the colorectal cancer. Methods:1: This study chose the cases with colorectal cancer that were confirmed by pathology at the Anorectal Hospital in Wuhan from November 2004 to August 2008. Through checking the medical record of these patients, some information were obtained and recorded in the predesigned questionnaire. The survey included demographic characteristics, clinical, pathological, laboratory indicators and some other features.2: The patients leaved the hospital were followed by telephone, in order to ascertain their survival time and prognosis outcome.3: The data was analyzed using SPSS 12.0 software: chi square test was used to compare the positive rates in different groups, Kaplan-Meier method to calculate survival rate, Log-rank test was to compare survival rate, and COX regression test to analysis factors related to survival time, so as to select independent prognostic factors.Results:1: There were no significant difference between the positive rate of CEA,LN and clinic features or pathological features (P>0.05). Difference of the positive rate of CA19-9 and tumor whole typing was statistically significant (P<0.05).2: There is significant difference in the combination of CEA and CA19-9 positive rate between the lymph node metastasis group and non-metastasis group, as to TNM stages and Dukes stages,â…¢/â…¢staging was higher thanâ… /â… staging and C/D staging than A/B staging (P<0.05). The positive rate of combination of CEA,LN related to the tumor whole typing was statistically significant (P<0.05), and that of the combination of three biomarkers positive rate were related to the cancer different type and Dukes stages (P<0.05).3:209 cases with integrity follow-up information were selected. The media follow-up time is 25 months. One-year, three-year and five-year survival rate of the CRC patients were 92.3%, 72.7% and 53.8% respectively.4: The Log-rank test results showed that the colorectal cancer patients'survival time was related to the following factors: tumor whole typing, differentiation degree, lymphatic metastasis, TNM classification, Dukes classification, post-operative chemotherapy, laboratory test results (including pre-operative serum level of CEA,CA19-9,the combination of CEA and CA19-9,the combination of CEA and LN, the combination of CA19-9 and LN,the combination of above the three biomarkers ).5:Post-operative chemotherapy, tumor Dukes'staging and the level of three tumor biomarkers in parallel were independent prognosis factors by COX regression test.Conclusion:1: This study only found that the combination of CEA,CA19-9 and LN positive rate related to CRC clinic/pathological factors were statistically significant. The relationship between single biomarker and clinic/pathological factors was not obvious in the study and further clinical or population research was needed to prove.2: High blood concentration of CEA,CA19-9 and the combination of the three biomarkers of colorectal cancer patients before resection pointed to poor prognosis, within which, the combination of the three biomarkers acted as independent prognosis factor. This study didn't find out the positive association between LN and the cases'prognosis.3: Dukes classification acted an important role in the colorectal cancer patients'prognosis, which suggested that it should be taken full consideration of this factor. |
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