Screening And Analysis Of Differentially Expressed Genes In Gastrocnemius Muscle Between DGAT1 Transgenic Mice And Wild Type Mice

Adipose tissue is the major energy deposition site of the mammalian, and provides the energy for the body and release the pressure from external to internal organs. In animal production, it also can affect meat quality traits, especially the intramuscular fat content and tenderness. Triacylglycerol is the important component of the adipose, and the DGAT1 is the key enzyme to control the synthesis of the triacylglycerol. The study of its mechanism is helpful to improve the content of intramuscular fat and the meat quality. In order to illuminate the new mechanism of DGAT1 for regulating the deposition of intramuscular fat, we detected the differential expressed genes in gastrocnemius between the muscle over expressed DGAT1 transgenic mice and wild-type mice using Affymetrix AFF-900623 mouse gene chip. Total cDNA of transgenic and wild-type mice was used to hybridize with gene chip, and the data were analyzed using Console Expression software. The results showed that 281 differential expressed transcripts were identified with fold change of at least 1.5 and p value of <0.05. Of 281 differentially expressed transcripts, 169 transcripts were up-regulated and 112 down-regulated in gastrocnemius. Ten genes(SREBF1, PDK4, UCP3, DUSP1, FKBP5, ZBTB16, PLAGL1, PPP1R3 C, CDC14 A and GLUL1) were selected to validate the reliability of the results of gene chips using QPCR, and the results of RT-PCR and gene chips were consistent. The signal pathway analysis of the differential expressed genes were conducted using MAS 3.0 and the pathway of differential expressed genes mainly focused on the G-protein coupled receptor protein signaling pathway, signal transduction, oxidation reduction, olfactory receptor activity, protein binding and zinc ion binding. These results provide theoretical basis for the mechanism of DGAT1 regulates content of the intramuscular fat.