The QTL Mapping Of Leaf Angle In Maize

Corn is one of the most important food crop, the production of corn in 2013 of our country is over 215 million tons, it has become China’s first major food crops. Domestic and international experience shows that improve the yields of maize production is main measures to promote the development of corn, while corn yield improvement is mainly dependent on the breeding of new varieties and updates. Compact plant breeding high-yielding hybrids, through rational close planting, so that the corn yield per unit area in an important way to improve. In recent years, with the rapid development of molecular genetic techniques quantity control several important traits in corn have been gene mapping, cloning, which will help research and improved varieties of maize genetics. SSR markers used in this experiment,in order to control the maize leaf angle QTL initial positioning.The 130 SSR markers have been divided into 10 linkage groups, covering 10 chromosomes of maize, the total map length was 1979.7cM, the average distance of 15.23cM. The No.1-10 Chromosome, with the length of 298.9,215.3cM,180.6cM, 252.9cM,216.9cM,215.2cM,170.2cM,173.4cM,147.9cM,108.5cM,have 27,13,6, 19,18,15,11,11,7,4 markers, respectively. The map is consistent with mazie genetic map, which has been published.This experiment depending on the average data of F2:3 family in mean leaf angle traits, has been in process of correlation analysis.It is clearly that three traits of mean leaf angle traits, on the level of 0.01,showde significant correlation. The corelation coefficient of Hoshita Hakazuho leaves,ear lobe and the inverse two leaf,ear leave and the inverse two leaf, was 0.588,0.396,0.391,respectively,in addition, the correlation coefficient of Hoshita Hakazuho leaves is the highest in all of three, that is to say,higher than the corelation coefficient of ear lobe and the inverse two leaf, the same as ear leave and the inverse two leafTaking advantage of composite interval mapping method to detect the micro QTL, controling leaf angle, located 7 QTL positionings related with leaf angle, initialy Three QTL locus, controlling spikle leave angle, located in the 1,6 chromosome; two QTL locus,dominating leaf blade angle, located in the 1,4 chromosome;the rest of two QTL locus, locating in the 2,8 chromosome, regulated the inverse two leaf blade angle. One major QTL site has been detected, in the between of bnlg1055 and umc1605 marker on chromosome 1, at the same time, in the ear leaf on the mountain was also detected in this QTL site.It is expected further study.4. Experiment location a QTL on No.8 chromosome locus near Umc1728, and Pengfei Liu (2002) Detection of consistency, and also detected QTL study sites located at 2.02 and previous position thought fit.In this study, positioning 7 QTL associated sites which is relative to leaf angle, to create conditions for closer study. On the basis of this study, can target QTL chromosomal regions where the increase of molecular markers for fine positioning of the target QTL.