| Potato(Solanum tuberosum L) is polyploidy crops. There are a great number of different ploidy germplasm in the nature. Diploid wild potato species are an important gene pool of resistant to biotic and abiotic stresses, and they have superior agronomic traits. The cultivated potato(Solanum tuberosum L) is highly heterozygous that tubers are asexual propagation and nutrient storage organs. The tuber is formed from top of expanded stolon and this process was regulated by environmental factors and endogenous regulators of regulation. The development characteristic of stolon was investigated and the influence factors were analyzed in different germplasm. Comparative proteomics between diploid wild potato species and the cultivated potato were investigated. The present research will reveals the mechanisms of stolon development and tuber formation. The research results will be basis of theory and practice for the utilization of potato germplasm resources.In this experiment, tetraploid species Atlantic, QS2( Qingshu2), KX2(Kexin2)FAV(Favorita), SHE(Shepody) were used as materials to determine and analysis the data of growth and development of stolon. Diploid species cv. W-6(S.goniocalyx), W-7(S.stenotonum), W-8(S.goniocalyx) and tetraploid species cv. Atlantic, QS2(Qingshu2), GN2(Gannongshu2) were used as materials to analysis comparatively proteins of potato tuber between diploid and tetraploid. The detailed results were as following:Potato stolon final length was depended on the start time point, growth rate and the stop time point of the stolon. The length of the stolon was depended on the growth period. The potato species of short growth period have a short time of stolon occurrence. The start time point of stolon was influenced by endogenous hormones which synthesized by over ground plant. However, the growth rate of stolon was not correlate with the final length of stolon under in vitro culture conditions. The present results also showed that the growth and development of stolon were regulated by the content of GA3.The comparative proteomics of tuber protein from diploids and tetraploids were analyzed. The result show that the particular protein of diploids include ADP-glucose pyrophosphorylase, UTP--glucose-1-phosphate uridylyltransferase, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase, cysteine protease inhibitor, Glucose-6-phosphate 1-dehydrogenase, catechol oxidase B, glutathione S-transferase,delta-1-pyrroline-5-carboxylate dehydrogenase. The functions of above proteins were related to drought resistance, salt resistance and other adverse environment resistance. The particular protein of tetraploid include p23 co-chaperone, mitochondrial small heat shock protein, glycine-rich protein 2, UTP-glucose-1-phosphate uridylyltransferase, class I chitinase, 17.6 k Da class I heat shock protein, proteasome, alpha subunit-like protein. The proteins of tetraploid indicated that they were related to resistance to heat, disease-resistant and other adverse environment resistance. The particular protein of GN2(2n×4n) was analyzed. The results show some proteins were involved in both diploids and tetraploids proteins. |
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