Effects Of Tributyrin And Coated Sodium Butyrate On Morphology, Disaccharidase And Energy Status Of Small Intestine In Acute Stressed Broilers

Experiments were conducted to investigate the effects of tributyrin and coatedsodium butyrate on morphology, disaccharidase and energy status of small intestinein lipopolysaccharide (LPS)-challenged broilers.160one-day-old Cobb chicks(average weight were42.33±0.89) g were randomly assigned into four groups:control group, LPS group, tributyrin (LPS+TB) group and coated sodium butyrate(LPS+CSB) group. Broilers in control group and LPS group were fed basal dietsupplemented with1000mg/kg silica. LPS+TB group were fed basal dietsupplemented with500mg/kg45%tributyrin and500mg/kg silica, whereasLPS+CSB group were fed basal diet supplemented with877mg/kg30%coatedsodium butyrate and123mg/kg silica. Thus, chicks in LPS+TB and LPS+CSBgroup both were fed diet with194.22mg/kg butyrate. Each group included6replicates,10chicken per replicate. On d22of the age body weight and feed intakewere recorded, and broilers of LPS, LPS+TB, and LPS+CSB groups wereintraperitoneally injected with1mg/kg·BW LPS, whereas the control group wereintraperitoneally injected with the same volumn of physiological saline.10chickswere killed and the intestinal samples were collected.The results showed that:1Effects of tributyrin and coated sodium butyrate on morphology of smallintestine in LPS broilersCompared with the control group, administration of LPS decreased villusheight and villus height/crypt depth, and increased crypt depth and villous width ofsmall intestine (P<0.05), but increased villous surface area of ileum (P<0.05). Dietsupplemented with TB increased duodenal villus height, duodenal and ileal villusheight to crypt depth ratio and ileal villous surface area (P<0.05), whereasdecreased duodenal and ileal crypt depth and villous width (P<0.05) in comparisonwith the control group. Diet supplemented with CSB increased villus height of duodenum and jejunum (P<0.05), the villus height to crypt depth ratio of duodenumand ileum (P<0.05), and villous surface area of ileum, whereas decreased cryptdepth of small intestine (P<0.05) and villous width of duodenum and ileum (P<0.05)compared with the LPS group. Villus height of duodenum and jejunum, crypt depthof jejunum, villus height to crypt depth ratio of duodenum and jejunum, villouswidth of small intestine did not differ between control and coated sodium butyrategroups (P>0.05). Compared with tributyrin group, supplemented with877mg/kg30%coated sodium butyrate significantly increased villus height of jejunum(P<0.05) and surface area of jejunum (P<0.05), and decreased crypt depth ofduodenum(P<0.05). These results showed that supplementation of TB or CSB caneffectively attenuated gut injury induced by LPS.2Effects of tributyrin and coated sodium butyrate on mucosal DNA and RNAcontents of small intestine in LPS-challenged broilersLPS administration decreased mucosal DNA content of duodenum and jejunum(P<0.05), decreased mucosal RNA contents of duodenum (P<0.05), and increasedTP/DNA of small intestine (P<0.05) in comparison with the control group. Dietarysupplementation of500mg/kg45%tributyrin increased DNA contents, RNAcontents and TP/DNA of duodenum (P<0.05). However, DNA content, RNA contentand TP/DNA of duodenum were not differed between control and tributyrin groups(P>0.05). Dietary supplementation of877mg/kg30%coated sodium butyrateincreased DNA content of duodenum (P<0.05), increased the ratio of RNA to DNAof jejunum (P<0.05). Compared with tributyrin group, coated sodium butyrategroup decreased RNA content of jejunum (P<0.05), decreased RNA/DNA ofduodenum (P<0.05), increased RNA/DNA of the jejunum (P<0.05). These datashowed that supplementation of500mg/kg45%tributyrin or877mg/kg30%coated sodium butyrate can alleviate the growth inhibition induced by LPS in thesmall intestine.3Effects of tributyrin and coated sodium butyrate on mucosal disaccharidasesactivities of the small intestine in LPS-challenged broilers LPS injection decreased the activity of maltase in jejunum (P<0.05) and theactivities of isomaltase and sucrase in the small intestine (P<0.05).Dietary supplementation of500mg/kg45%tributyrin increased the activityof maltase in jejunum (P<0.05), and the activities of isomaltase and sucrase induodenum and jejunum (P<0.05). The activity of sucrase in jejunum, as well as theactivities of isomaltase and sucrase did not differ between control and tributyringroups (P>0.05). Moreover, diet supplemented with877mg/kg30%coated sodiumbutyrate increased the activity of isomaltase in the small intestine (P<0.05), andincreased the activity of sucrase in jejunum (P<0.05). It could be concluded dietarysupplementation of500mg/kg45%tributyrin increased the disaccharidasesactivities of the small intestine in LPS-challenged broilers.Additionally, CSB diet decreased the activity of maltase in jejunum (P<0.05),and the activity of sucrase in duodenum and jejunum (P<0.05), whereas increasedthe activity of isomaltase in jejunum and ileum (P<0.05).4Effects of tributyrin and coated sodium butyrate on adenine nucleotideconcentrations of the small intestine in LPS-challenged broilersLPS stimulation decreased the intestinal ATP content and energy charge (EC)and ADP content in duodenum and jejunum (P<0.05), whereas increased the AMPlevels in jejunum and ileum (P<0.05). Dietary supplementation of500mg/kg45%tributyrin increased ATP and energy charge in duodenum and jejunum (P<0.05)compared with the LPS group. Similarly, dietary supplementation of877mg/kg30%coated sodium butyrate increased ATP and energy charge in duodenum andjejunum (P<0.05), and AMP concentrations in ileum (P<0.05) in comparison withthe LPS group. The results showed that supplementation of500mg/kg45%tributyrin or877mg/kg30%coated sodium butyrate can improve the intestinalmucosal energy status. In addition,there were no significant differences in theseparameters regarding the intestinal energy metabolism between these two groups.In summary, these data showed that diet supplemented with194.22mg/kgbutyrate improved the intestinal histomorphology, elevated the activity of mucosal disaccharidase, and preserved the energy metabolism in broilers. Therefore, dietarybutyrate can effectively alleviate intestinal mucosal injury in LPS-challengedbroilers.