Research On The Micropropagation Of Vaccinium Uliginosum Superior Lines SL-1

Vaccinium uliginosum belonging to Ericaceae Vaccinium as well as shrub,commonly known as bilberry,blueberry. The fruit is not only rich in nutrients,minerals and various vitamins,but also contains much anthocyanin,flavonoid and other physiological activity components. It helps relieve eye fatigue;prevent and treat hypertension and dredge capillary;Blueberry,by the international,is listed as one of the five major human health food [1-3].In this experiment,we used the Vaccinium uliginosum’s line SL-1 as materials,processed with ZT of different concentrations.for primary culture;use ZT,6-BA and IBA as 3 factors and 3 levels of L9(33)for orthogonal test to proliferate culture;using the leaves and stems of former material,different contents of NAA and 6-BA on callus induction and differentiation;Using different ratio and types of hormone and methods for Vaccinium uliginosum SL-1’s rooting culture. Study the rapid propagation of Vaccinium uliginosum’s excellent strain SL-1 in order to obtain a fast and efficient method to tissue culture,optimase the induction and differentiation of Vaccinium uliginosum excellent SL-1’s callus,improve the value of coefficient and the rooting rate of Vaccinium uliginosum excellent strain SL-1,short the time of Plantlets invitro rapid propagation, lay a foundation for research on Vaccinium uliginosumand SL-1 propagation, also provide theological and technology support for plant tissue culture in factory propagation.The results are as follows:(1) The optimal primary culture medium of Vaccinium uliginosum and SL-1 single bud stem explants ofoptimal is WPM+ZT1.0 mg/L + sucrose 20 g/L;Tube seedlings grow in a good condition;the survival rate was up to 80.3%;(2) Callus induction was found in leaves and stem of Vaccinium uliginosum SL-1,the leaves callus induction rate was the highest up to 100% when the NAA’s concentration was 0.8 mg/L, 6-BA’s concentration of 4 mg/L,the maximum rate of the stem segments is 86.7%; the leaf callus differentiation effect is the best when the concentration of NAA is 0.2 mg/L,the concentration of 6-BA was 4 mg/L,the differentiation rate reached 66.7%,the average number of regenerated plantlets were up to 5.765 strains;the stem regeneration differentiation rate was 54.2,the average number of adventitious buds 3.231 strains when the NAA’s concentration is 0.2 mg/L, the concentration of 6-BA was 4 mg/L;(3) The optimal medium composition of Vaccinium uliginosum excellent line SL-1’s proliferation is WPM+6-BA1.0 mg/L+NAA 0.1 mg/L+ZT1.5 mg/L+8 g/L agar and 20 g/L sucrose,the proliferation coefficient reaches 5.35;(4) Vaccinium uliginosum excellent line SL-1 the best effect of three rooting hormone’s combination was 1.5 mg/LIBA,2 mg/LIAA and 0.2 mg/L NAA,the rooting rate was 74.3%.(5) When directly added IAA in the culture medium,the rooting rate can up to 24.33% which was the highest when the concentration was 3 mg/L,the rooting effect is poor when the IAA concentration was 500 mg/L,the rooting rate was 80%,the average root number is 8.3;(6) When directly added IBA in the culture medium,the rooting rate was up to 68% when the concentration was 2 mg/L,accompanied by callus formation;when using the stem segment dipping IBA,the rooting rate was 100% when the IBA’s concentration was 300 mg/L,the average root number was 9.667,at the same time the root is strong;(7) Processing Vaccinium uliginosum SL-1 stems dipped in IBA of 300 mg/L to grow root, 10 s was the best time,the rooting rate is 100%;with the dipping time lengthened,accompanied by the burning phenomenon of seedling;the culture medium is WPM,MS,1/2WPM and 1/2MS, while the WPM is best for rooting,the rooting rate reached 100%.(8) The rooting effect differed when the dipping depth changed. The rooting rate was 100% when it was on the base of dipping,the average root number was 9.67.