The Study Of The Anti-inflammatory Activity Of The Essential Oil From Artemisia Argyi

[Objective] Inflammation is the defense reaction of the body to a variety of injuries, is a complex physiological and pathological processes. Most diseases are constantly accompanied by the inflammatory reaction and, therefore, the control and treatment of inflammation has a very important significance. So, we studied the anti-inflammatory activity of the essential oil from Artemisia argyi through different inflammatory models, and preliminary studied the anti-inflammatory mechanism, to providing a theoretical basis for the clinical application of the essential oil from Artemisia argyi.[Method] The essential oil from Artemisia argyi extracted by steam distillation, and the effects of the essential oil from Artemisia argyi were observed in early, middle and late inflammatory models, through the models of celiac capillary permeability of mice induced by acetic acid, auricle turgid of mice by xylene, edema of mice hind paw of mice induced by carrageenan, the chemotaxis of leucocyte induced by CMC, weight of granuloma in mice. While the effects of the essential oil from Artemisia argyi were studied on infiltration of PGE2、content of MDA and activity of iNOS in edema of mice hind paw induced by carrageenan.[Results]1. The essential oil from Artemisia argyi of the experimental extraction with bluish green, drug fragrant smell and transparent, and the oil yield was0.69%2. Compared with the blank control group, the low-dose group of the essential oil from Artemisia argyi had significantly inhibition effects (p<0.05), the high-dose group showed a extremely significant inhibition effects (p<0.01) on model of celiac capillary permeability of mice induced by acetic acid; both the low-dose and high-dose groups showed a extremely significant inhibition effects (p<0.01) on models of auricle turgid of mice by xylene and edema of mice hind paw of mice induced by carrageenan; the high-dose group showed significant inhibition effects (p<0.05) on models of the chemotaxis of leucocyte induced by CMC and weight of granuloma in mice.3. Compared with the blank control group, the high-dose group of the essential oil from Artemisia argyi had significantly inhibition effects (p<0.05) on infiltration of PGE2in edema of mice hind paw induced by carrageenan, and the low-dose group had certain inhibition effects, but no statistically significant; both the low-dose and high-dose groups showed a extremely significant inhibition effects (p<0.01) on content of MDA and activity of iNOS in edema of mice hind paw induced by carrageenan.[Conclusion] The results of inflammatory models showed that the anti-inflammatory effects of the essential oil from Artemisia argyi on all inflammation process (early, middle and late), and the anti-inflammatory mainly effected the early process of inflammation, especially for early inflammation showed strongly inhibitory effects in increased capillary permeability, tissue fluid exudation and swelling, to play an anti-inflammatory effect. The anti-inflammatory mechanism of the essential oil from Artemisia argyi in early process by inhibiting the production and release of PGE2, reduce the production of MDA and inhibition activity of iNOS to achieve.