Detection Of Transgenic TvNHX1Soybean Progenies And Analysis Of Saltandalkali Tolerance In Soybean

Soil salt-alkaline is a worldwide problem. Salt-alkaline has become one of themajor abiotic stress for the reduction of the global available arable land and therestrictions on crop yield. The development and utilization of saline land resources hasextremely important practical significance. Soybean (Glycine max) has a long plantinghistory in China. It is an important crop for food and feed industry and is also animportant source of human protein. Tripolium Vulgare TvNHX1gene has been cloned,transferred into soybean and obtained seeds of transgenic T2generation in our previousresearch work. This paper identified TvNHX1gene in transgenic soybean generationsand get T3and T4generation of transgenic soybean seeds. Meanwhile, the offspring ofgenetically modified soybeans were analyzed under salt-alkaline stress. The maincontents and results are as follows:Three transgenic soybean lines (T3generation) were indentified under200mmol/LNaCl stress by PCR. All T4generation were indentified under200mmol/L NaCl stressand PPT resistance by PCR,55transformed positive plants were obtained. The methodscould save a lot of time and funds in identifying the transformed positive plants.Expression of TvNHX1and GmNHX1gene in different T5transgenic soybean linesafter200mmol/L NaCl and200mmol/L NaHC03stress for5days was studied bysemi-quantitative RT-PCR. The results reveal that higher expression of TvNHX1under200mmol/L NaCl stress. The expression of GmNHX1genes under200mmol/L NaCl and200mmol/L NaHC03stress in22021-1and21066-2of transgenic soybean lines werehigher than unstress transgenic soybean.Content of MDA, activity of SOD, POD and CAT, and concentration of Na+, K+,Mg2+and Ca2+of different transgenic soybean lines are deternined under200mmol/LNaCl and200mmol/L NaHC03stress for5days. Under200mmol/L NaCl and200mmol/LNaHC03stress, the content of MDA was lowered, and the activity of SOD,POD and CAT were highered, which proves that21216-6has stronger salt-alkalinetolerance. Under200mmol/L NaCl and200mmol/L NaHC03stress, the concentration ofNa+of transgenic soybean roots was lower, and the concentration of K+, Ca2+and Mg2+ were remain stable. Keep ion homeostasis in balance. The TvNHX1gene plays animportant role in plant under salt-alkaline stress.