Estradiol Effects Of PGE₂and PGF_2α Receptors Expression In Bovine Oviduct Smooth Muscle

Estrogen (estradiol, E2) is a mammal of the hormones that regulate reproductive physiological process of important, one of the fallopian tube as an important part of the mammalian reproductive system, the connection between the two parts influences the whole reproductive physiological process. This study aims to elucidate estrogen for dairy cattle oviduct smooth muscle of prostaglandin receptors expression regulation function, explore the estrogen rhythmicity of fallopian tube smooth muscle contraction of sensitization effect.1. Bovine oviduct smooth muscle activity in vitro and identification of the organizationIn vitro isolation and culture of fresh dairy bovine oviduct smooth muscle tissue7d, after the identification of the active multi-channel physiological information acquisition system., according to the results of in vitro culture of oviduct smooth muscle tissue contraction in good condition, conform to the conditions in the experiment with the next step.2. Estradiol for dairy bovine oviduct smooth muscle tissue EP2, EP4and FP prostaglandin receptor mRNA expressionCultured dairy bovine oviduct activity of smooth muscle tissue and carries on the appraisal, using RT-PCR technique to detect EP2, EP4and FP prostaglandin receptor mRNA expression., according to the results of E2, under the effect of different concentration and time can make cows, in varying degrees, oviduct smooth muscle tissue EP2, EP4and FP prostaglandin receptor mRNA expression of the quantity increase, and E2for these three receptor mRNA expression of regulation is a regulation with concentration and time specific role3. Estradiol for dairy bovine oviduct smooth muscle tissue FP prostaglandin receptor protein expressionCultured dairy bovine oviduct activity of smooth muscle tissue and carries on the appraisal, gradient grouping in E2role in different time, respectively to extract the fallopian tube smooth muscle tissue of total protein, using Western blot technique to detect FP prostaglandin receptors expression of receptor proteins. Results show that the amount of FP receptor protein expression with E2effect increased with the extension of time.The experimental study on the main conclusions are as follows:(1) E2can promote oviduct smooth muscle tissue EP2, EP4and FP prostaglandin receptor mRNA expression.(2) E2can promote tubal FP prostaglandin receptor protein expression in smooth muscle tissue.