Application Research Of Interferon As Immune Regulator On Commercial Broiler Chicken

Avian influenza is an infectious disease of birds caused by type A influenza viruses, clinical manifestations of respiratory infections, drop in egg production, neck edema and diarrhea. H9subtype of avian influenza is low pathogenicity avian influenza, a small amount can cause death or not death, showed growth retardation and drop in egg production. Widely H9subtype avian influenza virus (AIV) in many countries in Southeast Asia including China and the world distribution of mainland China in1994for the first time publicly reported isolated from chickens to H9subtype AIV, then they form a small scale in some areas popular, not spread throughout the country. But the beginning of the spring and summer of1998, H9subtype of avian influenza in just a few months to form spread over20provinces nationwide pandemic, to China’s poultry industry caused huge economic losses.80years ago, people found the body could interference phenomenon for another virus after infection with a virus.But it was not until the late50s, Isaacs and Lindenmann used chicken embryos villi allantois membrane to study the flu interference phenomenon when it learned that the virus infected cells can produce a kind of factor, the effects on other cells, interfere with the virus replication, so named interferon(interferon, IFN). Interferon is a kind of important cytokines, it have extensive biological activities, and was produced by the body’s cells after the virus or other induce agent effect.It had immune regulation, cell proliferation, antiviral resistance and many kinds of biology and is an important part of organism defense system.This paper studies the influence of vaccine immune effect by mixed drink low-dose alpha-interferon in chickens (ChIFN-a), alpha-interferon (IFN-a) to healthy Chicken for Newcastle disease (NDV), avian influenza (AIV), infectious bronchitis vaccine (IBV), acute infectious bursal disease (IBDV). The large broiler farm is selected as the target test sites (number more than100,000), in young broiler until9th of age, randomly extracted3～5, meat chicken house, each number10,000sheds, set the experimental group and the control group.9-day-old immune Newcastle disease, avian influenza vaccine after48hours, the test group mixed drink one parts the dose ChIFN-alpha twice. And blank group, mixed drink tap water. Broilers mixed drink administered following pilot projects:1. commercial broiler farm Newcastle disease and avian influenza H9virus isolation: Through the different commodities broiler farm cotton swab sampling, sample processing, the allantoic cavity of9-day-old SPF chicken embryos inoculated with virus isolation, blind pass two generations, PCR methods detection of Newcastle disease and avian influenza H9viruses.2.The chicken recombinant interferon vaccine immune regulation:mixed to drink ChIFN-al4d after32d broiler humoral immune level.11-day-old broiler continuous mixed drink of1dose ChlFN-alpha (1times/day).at mixed drink14d,32D wing vein blood samples were taken, the use of Newcastle disease, avian influenza hemagglutination inhibition experiments, double sandwich ELISA method for the determination of serum IBV, IBDV antibody levels. The results showed that18days of age, the experimental group than the control group, the average value of more than0.3titer of avian influenza antibody levels than the control group, the average0.22titer uniformity between the experimental group of individuals than the control group;28age, experimental group Newcastle disease antibody levels than the control group average of more than a titer avian influenza antibody levels than the control group average of more than1.1titer uniformity between the experimental group of individuals than the control group;42days ND antibody levels in the experimental group than the control group average of0.34more titer antibody dispersion difference between uniformity in the experimental group of individuals than the control group, no difference between the two groups.;25days, the experimental group IBV, IBDV antibody more than the control group, the average0.25and0.37OD.3.Detection of different species immunomodulatory effects of interferon vaccine:11-day-old broilers were mixed drink of chicken recombinant the interferon ChIFN-alpha (experimental group Ⅰ) and recombinant interferon HuIFN-alpha (experimental group Ⅱ) immune14d broiler body fluidsimmunity levels. Experimental group,11-day-old broiler focus on a mixed drink doses ChIFN-alpha and HuIFN-alpha14, respectively, after the mixed drink14d wing vein to take blood samples to detect ND (HI), H9(HI). The results showed that the experimental group A, B Newcastle disease, avian flu antibodies were significantly higher than control group Ⅲ average antibody titers difference of up to two titer more, no significant difference between the experimental group A and B. And chicken interferon can significantly improve the Newcastle disease, avian influenza vaccine antibody levels.4.The effect of growth and development after mixed drink ChIFN-alpha. Each group were weighed at42days of age, the group as a unit to calculate the average daily gain for each treatment group.5.The results show that the mixed drink ChIFN-alpha on growth and development impact was not significant.These results suggest that broiler vaccines after1day, mixed to drink ChIFN-alpha high broiler antibody titers, immune effect, could enhance and regulate immune function, enhance disease resistance of broiler broiler. But no significant effect on the growth of broiler.