Dendrobium IR Qualitative And Quantitative Research

Dendrobii Officinalis Caulis is scarce traditional Chinese medicine, it has a good effect to human’s health, but its wild resources are rare, the current research on those medicinal material cultivation methods and the authenticity of identification are increasing in evidence. With the growing acreage, more species and varieties, the impact of its origin, age, and processing methods, these factors have not been given sufficient attention as a result it has indefinite quality and floated price level in the medicinal market, which has great inconvenience on the clinical application. The research collected Yunnan, Guangdong Guangxi, Zhejiang, Guizhou five main growing provinces domestic samples, using infrared spectroscopy combined with traditional chemical methods to the different parts, different years, different origin extracting, different drying types, different samples types of Dendrobium, while establishing a quantitative infrared calibration models polysaccharides, finally Dendrobium mineral element content were analyzed. The main findings are as follows:1. Dendrobium qualitative and semi-quantitative analysis of infrared spectroscopy in the impact of natural conditions.Analysis the IR of Dendrobium leaves, stems, roots and stems showed the polysaccharide similarity:stem> leaf> root, which was used to quick semi-quantitative analysis to the polysaccharide content. Second derivative spectra could be well resolved in the original spectrum overlapping peaks, the peaks in1318cm-1and782cm-1were intensity differences in the second derivative spectra, which displayed as calcium oxalate contents of leaf> stem>Polysaccharide content of different growth years for the Dendrobium:biennial> thirst> annual, and the correlation coefficient of the sample and the control map of polysaccharide content with their positive correlation. In the one-dimensional infrared spectroscopy could scan growth years of Dendrobium samples and the relative level of the polysaccharide content for quick identification.The polysaccharide contents between different origins had great differences:Zhejiang (47.90%), followed by Guangdong and Guangxi (42.84%,42.32%), Yunnan Dendrobium (13.55%) had lowest polysaccharide content, which were far below those from Zhejiang. The Dendrobium from five origins could use the second derivative infrared spectrum for identifying:in the peaks of1746cm-1(1745cm-1) and1738cm-1(1737cm-1), the Dendrobium from Yunnan and Guizhou had the almost same intensity peaks, while the samples from Zhejiang, Guangdong and Guangxi had the different peaks in the same areas. Whereby the Dendrobium from Yunnan, Guizhou could be separated from the rest of samples. The Dendrobium from Yunnan and Guizhou had the different peaks in the region of1515-1511cm-1, Yunnan’s samples had the almost same intensity peaks, the peak of1511cm-1was higher than the peak of1515cm-1in the samples of Guizhou, so the Dendrobium from Yunnan and Guizhoucould distinguish by each other. At the region of1563-1530cm-1, the IR of Dendrobium from Guangxi had two blunt peaks, while the samples from Guangdong and Zhejiang had four sharp peaks in different intensity, the differences could distinguish the samples from the three origins.2. Semi-quantitative and qualitative analysis of the Dendrobium infrared spectroscopy under the influence of human factorsThe content of polysaccharides had the differences between the oven-dried Dendrobium and freeze-dried Dendrobium, polysaccharide content in the freeze-dried samples was generally lower than the oven-dried samples. They were very similar in the one-dimensional infrared spectrum, and the correlations between them were high. Analysis the two dried types of Dendrobium in the two-dimensional infrared spectroscopy found the compositions of the polysaccharides and aromatic compounds were differed by each other.Analysis the IR of Dendrobium Officinale of Fengdous and Cunjin found Fengdous had the higher polysaccharide content while Cunjin had more aromatic compounds.Comparison the mineral elements content in different drying methods and pieces types of Dendrobium found, except titanium, each element and the total content of mineral elements of the freeze-dried samples were higher than dried samples; except chrome, each element and the total content of mineral elements of Fengdous were lower than Cunjin.3. Infrared quantitative calibration models of the Dendrobium polysaccharidesThe trials established the mid-infrared and near-infrared quantitative calibration models of Dendrobium polysaccharides, models used calibration standard deviation (SEE) for the optimization parameters, select the best principal components. The coefficient determination R2of the model were91.64%and99.83%, respectively, calibration standard deviation (SEE) values were3.034and0.4846, verification standard deviation (SEP) values were3.376and3.027, the average error of chemistry and predicted values were2.57%and2.51%, all of them indicated that the near-infrared and mid-infrared models were stable, reliable and the accuracy was good.