Electroacupuncture Hippocampus Of Depression-related Differences In Protein Expression Research CUMS Rats

Objective:Apply antibody microarray to detect hippocampal tissue of Chronic unpredictable mild stress rats, select the proteins which are significant difference in expression, disscuss the mechanism of depression which selected proteins might involved in, provide the experimental basis for discovering potential depression-related biomarker and clinical application of electric acupuncture treatment of depression.Methods:1. Forty Sprague-Dawley rats were randomly divided into control group, model group, electro acupuncture (EA) group and prozac group. The chronic unpredictable mild stress rat model was established,"Bai-Hui" and "Yin-Tang" points were used with EA, Prozac were used as positive control drug.2. Open field test, sucrose intake, and body weight balanee were used to evaluate the CUMS model.3. Collecting hippocampal tissues of control group, model group, electro acupuncture(EA) group and Prozac group to compare and analysis different proteins expression of4groups by Raybiotech Rat L-seriescytokine antibody chips.Results:1. The effect of electro acupuncture on rats’behavioristics. Before starting CUMS, there were no significant differences between each groups in squares crossed numbers、rearing-movement times、Sucrose consumption、body weight changes of rats (P>0.05). After chronic unpredictable mild stress of28days,Behavior change as follows:(1)In terms of squares crossed numbers, compared with control group,model group and prozac group had extremely significant difference (P<0.01),compared with model group,EA group and prozac group had extremely significant difference (P<0.01), there were no significantly differences between EA group and prozac group(P>0.05).(2)In terms of rearing-movement times,compared with control group,model group had extremely significant difference (P<0.01),compared with model group,EA group and prozac group had extremely significant difference (P<0.01), there were no significant differences between EA group and prozac group (P>0.05).(3) In terms of Sucrose consumption, compared with control group, model group, EA group and prozac group had extremely significant difference (P<0.01). Compared with model group,EA group and prozac group had extremely significant difference (P<0.01), there were no significantly differences between EA group and prozac group(P>0.05).(4) In terms of body weight changes of rats, compared with control group, model group, EA group and prozac group had extremely significant difference (P<0.01). compared with model group,EA group and prozac group had extremely significant difference (P<0.01). Compared with EA group, prozac group had significant difference (P<0.05).2. Compared with control group,30kinds of proteins which had greater than1.2-fold changes in expression were up-regulated,3kinds of proteins which had less than0.8-fold changes in expression were down-regulated in EA group. Compared with model group,4kinds of proteins which had greater than1.2-fold changes in expression were up-regulated,40kinds of proteins which had less than0.8-fold changes in expression were down-regulated inEA group. Compared with model group,3kinds of proteins which had greater than1.2-fold changes in expression were up-regulated,75kinds of proteins which had less than0.8-fold changes in expression were down-regulated in prozac group. EA group up regulate one kinds of the down-regulated proteins in model group while prozac group up regulate two. EA group down regulate23kinds of the up-regulated proteins in model group while prozac group down regulate26.3. Compared with control group,the proteins that differe-ntially expressed in EA group and prozac group were mainly down-regulated. Proteins that differentially expressed both in in EA group and prozac group were mainly down-regulated, involved in providing nutrion for neurons, angiogenesis, cell proliferation.cell differentiation and apoptosis, inflame-matory response, immunoregulation and Cell chemotaxis. Whereas, the up-regulated proteins involved in cell proliferation, cell differentiation and immuneoregula-tion.4. The proteins that different expressed in EA group or prozac group were mainly involve in JAK-STAT signal pathway、、 TGF-β/Smads signal pathway、MAPK signal pathway、NF-kB signal pathway and PI3/AKT signal pathway.5. Both EA group and prozac group can down regulate the up-regulated VEGF、 VEGF-C、b-FGF、TGF-β3and MMP13in model group. These proteins could promote nerve growth and angiogenesis, regulate the brain microenvironment.Conclusion: 1. EA can effectively reduce or prevent the occurrence of depressive behavio of CUMS rats. The effect of EA was similar to Prozac.2. The mechanism of EA and Prozac to treat depression was related to regulati multiple protein expression of hippocampal tissue, involved in several sign pathway. Differentially expressed proteins in EA group was relatively simil to Prozac group.3. VEGF、VEGF-C、b-FGF、TGF-β3and MMP13,differentially expressed protein were closely related to nerve regeneration and angiogenesis, indicating th EA and Prozac treat depression related to regulating the brain microenvironmen4. Comprared to prozac group, differentially expressed proteins of EA gro is more close to control group. Nimiety of down-regulated differential expressed proteins might caused by side effect of Prozac.